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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 534-547
DOI: https://doi.org/10.20546/ijcmas.2018.701.064


Phenotypic and Molecular Identification of Bacterial Species in Indian Major Carps and Exotic Carps from South 24 Parganas, West Bengal, India
Koel Bhattacharya Sanyal, Debapriyo Mukherjee, Ananya Guchhait and Gadadhar Dash*
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences 5, Budherhat Road, Chakgaria, P.O. Panchsayar, Kolkata, Pin-700094, West Bengal, India
*Corresponding author
Abstract:

The present study was done to find rapid and accurate identification of bacterial species which will help in prevention and cure of diseases in various aquatic animals. Phenotypic identification of bacterial isolates was done by conventional tests and commercial methods. Antibiotic sensitivity study revealed only gatifloxacin and chloramphenicol were found to be effective, both inhibited 83.33% sensitivity to the bacterial strains. Prevalence study revealed that aeromoniais were the dominant disease. The identities of selected bacterial strains were further confirmed by molecular characterization through 16S rDNA analysis. In 1.2% agarose gel electrophoresis, approximately 1.5 kbp bands were obtained by PCR amplification. In 2% agarose gel electrophoresis, approximately 675 bp and 1100 bp bands were obtained by PCR amplification for two Flavobacterium columnare specific primers set. Among the 7 isolates, 2 were identified as Commonas aquatica (accession number KT716080) and Aeromonas hydrophila (accession number KX455879) by using universal primers. Three isolates were identified as Flavobacterium columnare using specific primers by culture dependant and culture-independent methods. The sequence results of other bacterial isolates are awaited. The gene sequences of culture independent samples and culture dependent strains Commonas aquatica and Aeromonas hydrophila have been submitted to NCBI with accession numbers KX452118, KX452119, KT716080, KX455879, respectively.


Keywords: Antibiotic sensitivity, Exotic Carps, Indian Major Carps, NCBI, PCR
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How to cite this article:

Koel Bhattacharya Sanyal, Debapriyo Mukherjee, Ananya Guchhait and Gadadhar Dash. 2018. Phenotypic and Molecular Identification of Bacterial Species in Indian Major Carps and Exotic Carps from South 24 Parganas, West Bengal, India.Int.J.Curr.Microbiol.App.Sci. 7(1): 534-547. doi: https://doi.org/10.20546/ijcmas.2018.701.064