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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 506-510
DOI: https://doi.org/10.20546/ijcmas.2018.701.060


Studies on Thermo Stability of Newcastle Disease Viruses (Local Isolates) for Preparation of Vaccine
L. Sharma, U. Biswas, C. Guha, A. Chatterjee, P.S. Jana and R. Pandey
Department of Veterinary Epidemiology & Preventive Medicine, West Bengal University of Animal and Fishery Sciences 37, K. B. Sarani, Kolkata-700037, India
*Corresponding author
Abstract:

The study was carried out to assess the thermo stability of Newcastle Disease viruses (local isolates) which were isolated, characterised and provided by the Department of Veterinary Epidemiology and Preventive Medicine, West Bengal university of animal and Fishery Sciences. The thermo stability procedure was carried out at 250C for 36 days, 370C for 29 days, 400C for 30 days and 430C for 30 days respectively for each isolates. The results showed that all the five isolates tolerated 250C and 370C temperature for 36 days and 30 days respectively. The L4 isolate tolerated the 400C temperature for 18 days but the L5 isolate tolerated the 430C temperature for 12 days (showed HA titre 24 and EID50 107.5) was detected in 30 days heat exposure. The L5 isolate showed the longest viability after exposure to the highest temperature and can be used as seed virus for preparation of vaccine at room temperature after attenuation.


Keywords: Haemagglutination, EID50, Thermostability and Newcastle Disease Virus
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How to cite this article:

Sharma, L., U. Biswas, C. Guha, A. Chatterjee, P.S. Jana and Pandey, R. 2018. Studies on Thermo Stability of Newcastle Disease Viruses (Local Isolates) for Preparation of Vaccine.Int.J.Curr.Microbiol.App.Sci. 7(1): 506-510. doi: https://doi.org/10.20546/ijcmas.2018.701.060