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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 71-77
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.006


Hemolysin and Serum Resistance among ESBL Producing Extraintestinal Pathogenic Escherichia coli Isolated from a Tertiary Care Hospital
Sharath Kumar Shetty, D.T. Venkatesha, Sunil P Rao and K. Subbannayya
Department of Microbiology, Hassan Institute of Medical Sciences, Hassan, Karnataka, India
Department of Microbiology, Yenepoya Medical College, Yenepoya University Mangalore, Karnataka, India
Department of Microbiology, K.V.G Medical College, Sullya, Karnataka, India
*Corresponding author
Abstract:

Escherichia coli represent a major cause of morbidity and mortality worldwide. The treatment of Escherichia coli infections is now threatened by the emergence of antimicrobial resistance. The dissemination of resistance is associated with genetic mobile elements such as plasmids that may also carry other virulence determinants. The present study was undertaken to detect the virulence factors and drug resistance of extraintestinal pathogenic Escherichia coli. A total of 300 Extraintestinal pathogenic Escherichia coli isolated from various clinical samples and 50 commensal E. coli isolated from apparently healthy individuals were screened for virulence factors like serum resistance and hemolysin production. All Extraintestinal pathogenic Escherichia coli isolates were studies for antibiotic susceptibility pattern by modified Kirby-Bauer disc diffusion method. Extended spectrum lactamase (ESBL) production was screened by standard disc diffusion method and confirmed by CLSI phenotypic confirmation test. Among the 300 extraintestinal pathogenic Escherichia coli isolates 85 (28.33%) were hemolytic, 126 (42%) were resistant to bactericidal action of serum and 154 (51.33%) produced ESBL. The present study highlights the expression of virulence factors and multiple drug resistance in extraintestinal pathogenic Escherichia coli and the judicial use of antibiotics for management of their infections


Keywords: Escherichia coli, Haemolysin, Serum resistance, ESBL
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How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 71-77. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.006