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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 146-157
DOI: https://doi.org/10.20546/ijcmas.2018.701.016


Evaluation of Genetic Diversity in Some Banana Hybrids using ISSR Markers
Sukhen Chandra Das1*, T.N. Balamohan2, K. Poornima3 and I. Van Den Bergh4
1Horticulture, College of Agriculture, Tripura, India
2Department of Fruit Crop, HC and RI, TNAU, Tamil Nadu, India
3Nematology, Department of Nematology, HC and RI, TNAU, Tamil Nadu, India
4International Network for the Improvement of Banana and Plantain-Asia and the Pacific, c/o IRRI Khush Hall, Los Banos, Laguna 4031, Philippines
*Corresponding author
Abstract:

Bananas (Musa spp.) are mostly diploid or triploid with various combinations of genomes inherited from their ancestors. Inter simple sequence repeat (ISSR) markers were used for evaluation of genetic diversity of some synthetic Banana Hybrids. A total of 10 primers were evaluated for banana hybrids based on ISSR polymorphism. Diversity analysis carried out by using ISSR markers in some Banana hybrids revealed the confirmation of the hybridization along with the phylogenetic relationship of the hybrids with their parents. Out of the 10 primers studied, six primers exhibited scorable markers. A total of 54 markers were produced. The markers ranged from 5 to 8 in different primers. Of the 54 markers produced, 30 were polymorphic which account to 53.83 per cent polymorphism. Among the six primers studied ISSR 812 (UBC- 812) produced maximum number of polymorphic bands among the banana genotypes and might be useful for genetic diversity or DNA finger printing studies.


Keywords: Diversity, Hybrids, Markers, Cluster analysis, Polymorphism, ISSR, Dendrogram
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How to cite this article:

Sukhen Chandra Das, T.N. Balamohan, K. Poornima and Van Den Bergh, I. 2018. Evaluation of Genetic Diversity in Some Banana Hybrids using ISSR Markers.Int.J.Curr.Microbiol.App.Sci. 7(1): 146-157. doi: https://doi.org/10.20546/ijcmas.2018.701.016