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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 4309-4319
DOI: https://doi.org/10.20546/ijcmas.2017.612.495


Genetic Manipulation of Tomato (Solanum lycopersicum) cv. PKM-1 using cry2AX1 Gene for Insect Resistance
E.I. Bamishaiye, S. Varanavasiappan, N. Balakrishnan, V. Udayasuriyan and D. Sudhakar*
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India
*Corresponding author
Abstract:

Cotyledonary explants of tomato cv. PKM-1 were co-cultivated with Agrobacterium tumefaciens strain, LBA4404 harbouring a codon-optimised chimeric cry2AX1 gene driven by enhanced CaMV35S promoter in pCAMBIA2300 vector backbone. Ninety six putative transgenic plants were regenerated, and the presence of the cry2AX1 gene in fifty eight plants was demonstrated by PCR analysis. ELISA showed that nine out of the fifty eight plants had detectable level of Cry2AX1 protein expression, which ranged from 0.030 to 0.388 µg/g of fresh tissue. Insect bioassay of transgenic T0 tomato plants using H. armigera neonates recorded a mortality of 16.67 to 100 per cent and showed significant reduction in leaf feeding and inhibition of growth in surviving larvae. The results demonstrated the potential of the chimeric cry2AX1 gene in developing H. armigera resistant transgenic tomato varieties.


Keywords: Agrobacterium, Tomato transformation, Cry2AX1, ELISA, Insect bioassay, Helicoverpa armigera.
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How to cite this article:

Bamishaiye, E.I., S. Varanavasiappan, N. Balakrishnan, V. Udayasuriyan and Sudhakar, D. 2017. Genetic Manipulation of Tomato (Solanum lycopersicum) cv. PKM-1 using cry2AX1 Gene for Insect Resistance.Int.J.Curr.Microbiol.App.Sci. 6(12): 4309-4319. doi: https://doi.org/10.20546/ijcmas.2017.612.495