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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 4235-4247
DOI: https://doi.org/10.20546/ijcmas.2017.612.488


Antibacterial Activity of Lactic Acid Bacteria against Helicobacter pylori Evidence by in vivo and in vitro Studies
Nesreen Mohammed Nasr1*, Manal Khider1, Wedad Metry1 and Khaled Atallah2
1Department of Dairy Science, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
2Deptartment of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
*Corresponding author
Abstract:

Comparative studies on some probiotic potential of two selected strains of Lactobacillus were carried out during this work. Eight isolates of lactic acid bacteria were tested; the most effective isolates (LAB11 and LAB13) against Helicobacter pylori were selected for further probiotic properties and in vivo. The histological features of gastritis and the presence of H. pylori were also examined. The isolates LAB13 andLAB11 showed highly antibacterial activity which indicated by the largest zone diameter (50.0 and 49.0 mm, respectively). Based on tested probiotic properties the results of acid and bile salt tolerance showed a survival of both isolates with a significant (p<0.001) resistant for LAB13 more than LAB11. The gastric mucosa sections showed that rats which treated with probiotic isolate; LAB11 or LAB13 daily for a week before infection by H. pylori, and then infected rats treated with the same isolate (LAB11) for another 6 weeks (Group 3 and 5, respectively) and rats infected first by H. pylori and after 4 weeks of infection treated with LAB11 for 6 weeks (Group 4) showed negative H. pylori colonization. So it is recommended using dairy products containing probiotic bacteria as daily protective routine to eradicate growth or colonization of H. pylori.


Keywords: Lactic acid bacteria, Probiotics, Antibacterial activity, Helicobacter pylori, Gastric ulcers, Duodenal ulcers, Acid and Bile tolerance, Animal model.
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How to cite this article:

Nesreen Mohammed Nasr, Manal Khider, Wedad Metry and Khaled Atallah. 2017. Antibacterial Activity of Lactic Acid Bacteria against Helicobacter pylori Evidence by in vivo and in vitro Studies.Int.J.Curr.Microbiol.App.Sci. 6(12): 4235-4247. doi: https://doi.org/10.20546/ijcmas.2017.612.488