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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 3883-3898
DOI: https://doi.org/10.20546/ijcmas.2017.612.449


Isolation, Characterization and Antagonistic Activity of Fluorescent Pseudomonads
Priyanka1*, Geeta Goudar2, P. Jones Nirmal Nath1 and P.V. Patil3
1Department of Agriculture Microbiology, AC, Dharwad, UAS, Dharwad, Karnataka, India
2Department of Agriculture Microbiology, AC, Vijayapura, UAS, Dharwad, Karnataka, India
3Department of Plant Pathology, AC, Dharwad, UAS, Dharwad, Karnataka, India
*Corresponding author
Abstract:

An attempt was made to isolate 62 strains of fluorescent pseudomonads from rhizosphere of soybean collected from two soybean growing areas viz., Belagavi and Dharwad districts of Karnataka. These isolates were subjected for morphological, biochemical and functional characterization. All the isolates were gram negative rods. Colour of the colony varied from light green, green, dark green to light orange. The isolates showed fluorescens under UV light. Out of 62 isolates 36, 24, 52, 36, 39 isolates were positive for starch hydrolysis, arginine hydrolysis, oxidase test, denitrification and gelatin liquification respectively. These isolates exhibited wide variations for their ability to utilize different carbon sources. With respect to MPS, 52 isolates showed MPS activity. Forty seven isolates were positive for HCN production, in which 10 of the isolates were strong HCN producers. All the isolates were positive for siderophore production, which ranged between 6.00 to 36.37mm. Whereas, IAA and GA production ranged from 39 to 28.03 and 0.5 to 18.52 mg /25 ml of broth respectively. All the 62 isolates were assayed for their antagonistic activity against Sclerotium rolfsii and Colletotricum truncatum using dual plate technique. Out of 62 isolates, 51 and 38 were inhibitory to S. rolfsii and C. truncatum respectively.


Keywords: Fluorescent pseudomonads, Sclerotium rolfsii, Colletotricum truncatum, PGPR.
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How to cite this article:

Priyanka, Geeta Goudar, P. Jones Nirmal Nath and Patil, P.V. 2017. Isolation, Characterization and Antagonistic Activity of Fluorescent Pseudomonads.Int.J.Curr.Microbiol.App.Sci. 6(12): 3883-3898. doi: https://doi.org/10.20546/ijcmas.2017.612.449