National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In recent years, production of voluminous agricultural waste creates a major problem and is of environmental concern. Hence, there is great urge to adopt a reuse of agricultural waste. The objective of this study was to isolate cellulose and lignin degrading bacteria which are able to degrade agrowaste and convert agrowaste into green manure. Total of 103 bacteria were isolated. All these isolates were screened for their cellulase production by CMC agar plate assay. Out of which 51 bacterial isolates showed zone of clearance on CMC agar plates. 14 bacterial isolates which showed higher clearance zone on CMC agar plate were further selected for quantitative screening. Isolate 53 produced higher cellulase enzyme 0.636 U/ml. For screening of lignin degradation, ABTS plate assay was used for laccase enzyme. Out of all the 103 isolates, 10 showed positive result on ABTS agar plate. Among these 10 isolates, 4 isolate selected for further quantitative assay for Lignin Modifying Enzymes (LME) i.e., laccase, LiP and MnP. Isolate 72 showed higher laccase (453.6), LiP (95.0) and MnP (526.35) enzyme. Growth was also monitored upto 96 hrs of incubation during enzymatic studies. Two potential cellulolytic (isolate 53) and lignolytic (isolate 72) isolate were identified by 16 rDNA as Bacillus licheniformis C1 and Bacillus sp. L2, respectively.
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