Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 3808-3816
DOI: https://doi.org/10.20546/ijcmas.2017.612.438


Exploring Microbes for their Cellulolytic and Lignolytic Enzyme Activity for Manure Preparation
Neethu T. Madhavan1, K.G. Patel1, Trupti K. Vyas1* and Sree Ganesh2
1Food Quality Testing Laboratory, Navsari Agricultural University,Navsari – 396450, Gujarat, India
2Department of Genetics and Plant breeding, Navsari Agricultural University, Navsari- 396 450
*Corresponding author
Abstract:

In recent years, production of voluminous agricultural waste creates a major problem and is of environmental concern. Hence, there is great urge to adopt a reuse of agricultural waste. The objective of this study was to isolate cellulose and lignin degrading bacteria which are able to degrade agrowaste and convert agrowaste into green manure. Total of 103 bacteria were isolated. All these isolates were screened for their cellulase production by CMC agar plate assay. Out of which 51 bacterial isolates showed zone of clearance on CMC agar plates. 14 bacterial isolates which showed higher clearance zone on CMC agar plate were further selected for quantitative screening. Isolate 53 produced higher cellulase enzyme 0.636 U/ml. For screening of lignin degradation, ABTS plate assay was used for laccase enzyme. Out of all the 103 isolates, 10 showed positive result on ABTS agar plate. Among these 10 isolates, 4 isolate selected for further quantitative assay for Lignin Modifying Enzymes (LME) i.e., laccase, LiP and MnP. Isolate 72 showed higher laccase (453.6), LiP (95.0) and MnP (526.35) enzyme. Growth was also monitored upto 96 hrs of incubation during enzymatic studies. Two potential cellulolytic (isolate 53) and lignolytic (isolate 72) isolate were identified by 16 rDNA as Bacillus licheniformis C1 and Bacillus sp. L2, respectively.


Keywords: Biodegradation, Bacillus, Cellulase, Laccase, LiP.
Download this article as Download

How to cite this article:

Neethu T. Madhavan, K.G. Patel, Trupti K. Vyas and Sree Ganesh. 2017. Exploring Microbes for their Cellulolytic and Lignolytic Enzyme Activity for Manure Preparation.Int.J.Curr.Microbiol.App.Sci. 6(12): 3808-3816. doi: https://doi.org/10.20546/ijcmas.2017.612.438