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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 3407-3416
DOI: https://doi.org/10.20546/ijcmas.2017.612.396


Biochemical and Antimicrobial Characterization of an Underexploited Medicinal Plant - Verbesina encelioides
C.K. Divya Ramakrishnan1*, Dayal Doss2 and A. Vijayabharathi3
1Department. of Biotechnology, Sri Narayanaguru College, Coimbatore–641 105,Tamil Nadu, India
2Department of Plant Biotechnology, GKVK, UAS, Bangalore – 560065, Karnataka, India
3Department Genetics and Plant Breeding, University of Agricultural Sciences,
Bangalore – 560065, Karnataka, India
*Corresponding author
Abstract:

Verbesina encelioides is an exotic medicinal plant widely used in traditional medicine. Antimicrobial proteins are low molecular weight compounds involved in constitutive or induced resistance to microbial attack. The search for new antimicrobial substances exhibiting minimal side effect is warranted. The study was conducted to estimate some of the biochemical parameters and characterize the antimicrobial proteins from the young leaves of Verbesina encelioides. Plant was surveyed in Eastern dry zones of Karnataka and sample from each places were collected. Among them sample collected from Kolar (Thavarekere village) was found to have maximum total protein, sugar, phenol, tannin and micro-nutrients. SDS-PAGE analysis estimated the low molecular weight protein found to be of 14 k Da which is responsible for the antimicrobial activity. Bioassays were done to test the antibacterial and antifungal activity. Antibacterial activity was determined against selected bacterial pathogens like Escherichia coli, Enterococcus faecalis, Staphylococcus coagulance, Staphylococcus aureus, Pseudomonas aeruginosa. Later antifungal activity was determined by Aspergillus flavus, Rhizactonia solani, Fusarium oxysporum and Sclerotium rolfsii. The result confirmed that the protein exhibits moderate antimicrobial activity. This determines the relevance of antimicrobial activity of Verbesina encelioides.


Keywords: Verbesina encelioides, Antimicrobial, Antifungal, Biochemical characterization.
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How to cite this article:

Divya Ramakrishnan, C.K., Dayal Doss and Vijayabharathi, A. 2017. Biochemical and Antimicrobial Characterization of an Underexploited Medicinal Plant - Verbesina encelioides.Int.J.Curr.Microbiol.App.Sci. 6(12): 3407-3416. doi: https://doi.org/10.20546/ijcmas.2017.612.396