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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 3326-3332
DOI: https://doi.org/10.20546/ijcmas.2017.612.387


Optimization of a Novel Alkaline Protease from Cashew Industrial Soil Associated Aspergillus niger
G. Parvathy*, C. Prabhakumari, Nair Sreecha Chandran and Sonia John
Department of Biotechnology, CEPC Laboratory and Research Institute, Mundakkal, Kollam, Kerala, India
*Corresponding author
Abstract:

Protease the leading industrial enzyme accounts for about 60% of the total enzyme market. It has wide application in different sectors. The search of proteases with better activity and potential of different application is very important. The current paper screened cashew nut processing units’ soil near Kollam for the isolation of effective protease producers. The isolated fungal strains isolated from soil by serial dilution method were screened by casein hydrolysis for alkaline protease production. Out of the ten isolated strains of fungi A, B, G and H are protease producers. After the optimization the isolate strain H showed highest activity in the optimized medium at pH 11.0, temperature 35ºC, with glucose as carbon source incubated for 7 days. The highest enzymatic activity is showed in the 4th day of batch fermentation at 96hr of incubation. The most potent protease produced strain was identified based on morphological characterization characteristics in National Fungal Culture Collection of India (NFCCI & FIS) Pune as Aspergillus niger. Proteases represent one of the largest groups of industrial enzymes and find application in detergents in the removal of different kinds of stains and also in dehairing process practised in leather industry.


Keywords: Protease, Aspergillus niger, NFCCI.
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How to cite this article:

Parvathy G., C. Prabhakumari, Nair Sreecha Chandran and Sonia John. 2017. Optimization of a novel alkaline protease from Cashew Industrial soil associated Aspergillus niger.Int.J.Curr.Microbiol.App.Sci. 6(12): 3326-3332. doi: https://doi.org/10.20546/ijcmas.2017.612.387