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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 3191-3207
DOI: https://doi.org/10.20546/ijcmas.2017.612.373


A Review on Molecular Tools of Microalgal Genetic Transformation and their Application for Overexpression of Different Genes
Jayant Pralhad Rathod1*, Rajendra M. Gade1, Darasing R. Rathod2 and Mahendra Dudhare1
1Vasantrao Naik College of Agricultural Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Waghapur Road, Yavatmal-445001, Maharashtra, India
2Dr. Panjabrao Deshmukh Krishi Vidyapeeth, P.O. Krishi Nagar, Akola-444104,Maharashtra, India
*Corresponding author
Abstract:

Microalgae are in focus because of its tremendous potential to produce different value added compounds such as pigments, polysaccharides, cosmetics, fatty acids, proteins, vitamins and so on. Genetic engineering of microalgae promises broader applications in the field of molecular farming for the production of above mentioned industrially valuable compounds. For transformation of any of these gene widely used molecular tools such as particle bombardment, Agrobacterium mediated transformation, electroporation, and less commonly used Conjugation, Agitation with glass beads, Agitation with silicon carbon whiskers, Protoplast transformation with polyethylene glycol and dimethyl sulfoxide are used. The basic mechanism of these methods is to somehow disturb the cell wall and cell membrane of the algal cell and allow the gene to reach the nucleus via nuclear pore to integrate into the nucleus. Using above mentioned methods genes responsible for lipids, terpenoids, carotenoids and other different therapeutic recombinant proteins have been overexpressed. There are different challenges and existing problems for transformation and integration of genes in microalgae which needs to be overcome to fully utilize the potential of this microbe for molecular cloning and overproduction of different value added compounds.


Keywords: Microalgae, Particle bombardment, Agrobacterium mediated transformation, Electroporation, Overexpression, Cloning.
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How to cite this article:

Jayant Pralhad Rathod, Rajendra M. Gade, Darasing R. Rathod and Mahendra Dudhare. 2017. A Review on Molecular Tools of Microalgal Genetic Transformation and their Application for Overexpression of Different Genes.Int.J.Curr.Microbiol.App.Sci. 6(12): 3191-3207. doi: https://doi.org/10.20546/ijcmas.2017.612.373