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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2960-2967
DOI: https://doi.org/10.20546/ijcmas.2017.612.345


First Report of Whitefly Transmitted Begomovirus Infecting Chilli in Sub-Himalayan Zone (Cooch Behar) of West Bengal, India
Uday Bikash Oraon1*, Lourembam Sanajaoba Singh1 and Jayanta Tarafdar2
1Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia-741252, West Bengal, India
2AICRP on Tuber crops, Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia-741235, West Bengal, India
*Corresponding author
Abstract:

Chilli leaf curl disease, caused by the begomovirus (family- Geminiviridae), is an economically important disease of chilli (Capsicum annum L.) that can be very destructive in tropical and subtropical regions. In July- august 2015, upward leaf-curling, distortion, vein yellowing or more generalized leaf yellowing and/or stunted growth with bushy appearance with high abundance of vector whitefly (Bemisia tabaci) observed in chilli plants in the area of Cooch Behar, a diverse agroclimatic zone of sub Himalayan West Bengal, India. The incidence begomovirus infection on chilli was observed up to an extent of 20-90%. Presence of begomovirus infection was confirmed through Polymerase chain reaction (PCR) using begomovirus specific degenerate primer SPG1/SPG2. PCR product of 920 bp was detected, partially sequenced and has been deposited in NCBI (GeneBank Accession No. LT622252). The BLASTn analysis was performed with other begomovirus isolates in the GeneBank database of NCBI. The replication associated protein (AC1) gene of the virus showed highest 94% nucleotide sequence identity (nsi) with both Tomato leaf curl Joydebpur virus clone Moh9, complete genome [GeneBank Acc. No.- KJ028211] and Tomato leaf curl Joydebpur virus clone HJP09 segment DNA-A, complete sequence [GeneBank Acc. No. JQ654463]. This is the first report of a Begomovirus infecting chilli (Capsicum annum L.) in sub-Himalayan regions of West Bengal.


Keywords: Chilli, Begomovirus, Whitefly, PCR, BLASTn.
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How to cite this article:

Uday Bikash Oraon, Lourembam Sanajaoba Singh and Jayanta Tarafdar. 2017. First Report of Whitefly Transmitted Begomovirus Infecting Chilli in Sub-Himalayan Zone (Cooch Behar) of West Bengal, India.Int.J.Curr.Microbiol.App.Sci. 6(12): 2960-2967. doi: https://doi.org/10.20546/ijcmas.2017.612.345