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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2749-2757
DOI: https://doi.org/10.20546/ijcmas.2017.612.319


Biochemical Constituents Imparting Resistance to Sucking Pest Aphid in Cotton (Gossypium spp.)
T.C. Divya1, I.S. Katageri2*, M.P. Jadhav2, Sateesh Adiger2, H.M. Vamadevaiah1 and Nagaratna S. Olekar1
1Department of Biochemistry
2Department of Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India
*Corresponding author
Abstract:

Present investigation was performed to elucidate the role of selected plant antioxidant enzymes (lipoxygenase and nitrate reductase) and phenolic compounds (total phenol and condensed tannin) in imparting the resistance/susceptibility to aphid infestation in two cotton genotypes (CPD14-1 and CPD14-2). Leaf samples were collected from both aphid infected as well as uninfected plants at 45, 85 and 125DAS (days after sowing) and analysis of the phenolic compounds and enzymatic activities were carried out. Changes in the enzyme activity level were determined at different time interval after infestation. The results indicated that aphid infestation increased the total phenols, condensed tannin, and enzymatic activities. The resistant genotype CPD14-1 recorded higher phenol content (7.07g/100g dry weight), condensed tannin (7.97g/100g dry weight) and enzymatic activities lipoxygenase (3.42U/mg of protein) and nitrate reductase (62.6 nmoles of NO2 g/h) than susceptible genotype CPD14-2. The results suggested that enhanced activities of enzymes and phenolic compounds may contribute bioprotection of cotton plants against aphid infestation.


Keywords: Cotton, Sucking pest, Total phenols, Condensed tannin, Lipoxygenase and nitrate reductase.
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How to cite this article:

Divya, T.C., I.S. Katageri, M.P. Jadhav, Sateesh Adiger, H.M. Vamadevaiah and Nagaratna S. Olekar. 2017. Biochemical Constituents Imparting Resistance to Sucking Pest Aphid in Cotton (Gossypium spp.).Int.J.Curr.Microbiol.App.Sci. 6(12): 2749-2757. doi: https://doi.org/10.20546/ijcmas.2017.612.319