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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:12, December, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2708-2715
DOI: https://doi.org/10.20546/ijcmas.2017.612.314


Biosurfactant Production by Cunninghamella phaeosphora UCP 1303 Using Controlled Temperature Through of Arduino
Amanda B. Lins1, Ana P. Bione1, Tainã C.S. Fonseca1, Thaís C. Silva2, Paulo H. da Silva2, Kyriale V. Morant3, Rosileide F.S. Andrade4 and Galba M. Campos-Takaki5*
1Enviromental Process Development, Catholic University of Pernambuco,
50.050-900 Recife, PE, Brazil
2Chemical Engineering, Catholic University of Pernambuco, 50.050-900 Recife, PE, Brazil
3Chemical Engineering, São Paulo University (USP), 05508-900 São Paulo, SP, Brazil
4National Postdoctoral Program (PNPD-CAPES) - Postgraduate Programm in Development of Environmental Processes, Catholic University of Pernambuco, Recife, PE, Brazil
5Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50.050-590 Recife, PE, Brazil
*Corresponding author
Abstract:

The objective of this study was to investigate the ability of Cunninghamella phaeosphora UCP 1303 to produce biosurfactant converting soybean oil and corn liqueur, using temperature monitoring through the Arduino and DS18B20 waterproof temperature sensor. Initially, a 107 cells/mL suspension was inoculated and incubated under 150 rpm shaking at 25, 28 and 35°C during 96 h. Surface and interfacial tensions were determined by the Du Nouyring method and also the emulsification index and the dispersion test in water. The results demonstrated unprecedented use of Arduino in fermentation temperature control, with temperatures of 25°C and 35°C showing a surface tension reduction of 70 mN/m to 36.1mN/m and 70 mN/m for 37.1 mN/m respectively. Fermentation temperature at 28°C presented the best results for reducing surface tension (70 mN/m to 28.1 mN/m) and interfacial tension (2.2 mN/m) with high potential of motor oil emulsion Burned (E24 = 100%) and excellent dispersant properties (28.26 cm2) of motor oil burnt in water. The results showed that C. phaeospora produced biosurfactant in low cost medium using agroindustrial residues; in addition, the excellent properties of the biosurfactant suggest its potential of application in the bioremediation of sites polluted by hydrophobic compounds.


Keywords: Soybean oil, Corn steep liqueur, Agroindustrial wastes, Arduino, DS18B20 sensor.

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How to cite this article:

Amanda B. Lins, Ana P. Bione, Tainã C.S. Fonseca, Thaís C. Silva, Paulo H. da Silva, Kyriale V. Morant, Rosileide F.S. Andrade and Galba M. Campos-Takaki. 2017. Biosurfactant Production by Cunninghamella phaeosphora UCP 1303 Using Controlled Temperature Through of Arduino.Int.J.Curr.Microbiol.App.Sci. 6(12): 2708-2715. doi: https://doi.org/10.20546/ijcmas.2017.612.314
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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