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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2698-2702
DOI: https://doi.org/10.20546/ijcmas.2017.612.312


Study of Bacteriological Profile on Hands of Health Care Workers in ICU Setup in a Tertiary Care Hospital
Rincy Susan Mathew, R. Ambica and G.B. Shantala*
Department of Microbiology, Victoria Hospital Campus, Fort Road, Bengaluru – 560002, Karnataka, India
*Corresponding author
Abstract:

The most common channels for spread of Health care associated infections (HCAI) resulting in cross transmission are healthcare workers’ (HCW) hands. The aim of this study was to assess the bacteriological profile on hands of the health care workers working in ICU setup during patient care. Imprints of fingertips and palms from the participant's hands were taken during their routine patient care onto 5% sheep blood agar plate and MacConkey agar plate. After incubation at 37ºC for 24 hours, the bacterial colonies were identified using standard biochemical reactions. Staphylococcus colonies were checked for methicillin resistance. 88.33% of samples had growth on their plates of which 43.39%had only the resident flora of the hand. 41.67% samples had Staphylococcus colonies on their plates, of which 16.67% were found to be Staphylococcus aureus and rest 25% were CoNS species. 06.67% of the Staphylococcus aureus isolates were resistant to cefoxitin and 05% of the CONS were methicillin resistant. 9.43% of the samples yielded gram negative bacilli on their plates, out of which 5.6% were identified as Escherichia coli and 03.7% as Klebsiella species. Both the species were multidrug resistant. Hand contamination progressively increased during routine patient care; pointing towards the unsatisfactory hand hygiene practices in the ICUs. The importance of simple barrier precautions must be highlighted through constant training of staff for their wholehearted compliance.


Keywords: Bacteriological profile, HCW, Hands, ICU, MRSA.
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How to cite this article:

Rincy Susan Mathew, R. Ambica and Shantala, G.B. 2017. Study of Bacteriological Profile on Hands of Health Care Workers in ICU Setup in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 6(12): 2698-2702. doi: https://doi.org/10.20546/ijcmas.2017.612.312