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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2495-2503
DOI: https://doi.org/10.20546/ijcmas.2017.612.290


In-Vitro and In-Vivo Pathogenicity Study of Cryptococcus Species
A. Banerjee1, S. Pradhan1*, C. Debnath2, S. Baidya3, N.C. Patra1, R.N. Hansda1
S. Mondal1 and S.K. Mukhopadhayay1
1Department of Veterinary Pathology
2Department of Veterinary Public Health
3Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences,WBUAFS, Kolkata-700037, India
*Corresponding author
Abstract:

Cryptococcus neoformans encapsulated yeast causes localized or disseminated infections, which may be acute, sub-acute or chronic in nature, in immunocompromized and apparently immunocompetent patients. Unfortunately, knowledge concerning the genetic variability in relation to the pathophysiology of the disease remains controversial. Random Amplification of Polymorphic DNA (RAPD) technique is a novel molecular approach for detection of genetic variability of fungal strains. A RAPD technique using arbitrary primers R28, OPH-02 and OPH-20 clearly indicates genetic variability in C. neoformans. To find out relationship between genetic variability and pathogenicity of the C. neoformans, we carried out the in- vitro and in-vivo pathogenicity test in mice model, infected with four different RAPD types of Cryptococci. Mice showed a high susceptibility to Pattern I and Pattern IV of C. neoformans. This variation in susceptibility is associated with some minute difference in their genotypes but not related with the in-vitro virulence factors i.e. urease activity and melanin production.


Keywords: Patho-physiology, RAPD, Genetic variability, Pathogenicity.
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How to cite this article:

Banerjee, A., S. Pradhan, C. Debnath, S. Baidya, N.C. Patra, R.N. Hansda, S. Mondal and Mukhopadhayay, S.K. 2017. In-Vitro and In-Vivo Pathogenicity Study of Cryptococcus Species.Int.J.Curr.Microbiol.App.Sci. 6(12): 2495-2503. doi: https://doi.org/10.20546/ijcmas.2017.612.290