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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2443-2453
DOI: https://doi.org/10.20546/ijcmas.2017.612.283


Clostridium perfringens Type A from Broiler Chicken with Necrotic Enteritis in Kashmir Valley, India
Q. Nyrah1*, S.A. Wani1, N. Nazir1, S. Rasool1, Q. Beigh1, Z.A. Kashoo1, I.Hussain1, Sabia Qureshi1 and R. Ali2
1Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, India
2Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, India
*Corresponding author
Abstract:

The current study reports occurrence and molecular toxinotyping of Clostridium perfringens isolates in Kashmir valley. A total of 217 feacal samples and intestinal contents of broiler chickens suspected for enteritis were collected and screened for C. perfringens out of which 66 (30.41%) were found positive using Robertson’s cooked meat media and Sulphite Polymixin Sulphadiazine (SPS) agar. The isolates were confirmed by 16S rRNA gene specific PCR and analyzed by multiplex PCR for toxinotyping. All the isolates were found to harbour alpha toxin gene (cpa) of C. perfringens. However, 13 isolates also carried beta2 toxin gene (cpb2). None of the isolates were positive for beta (cpb), epsilon (etx), iota (itx) and enterotoxin (cpe) genes. Highest prevalence was found in chicken with age group of 2-6 weeks. Mortality ranged from 2-4 percent per day. Necropsy of chickens revealed that their intestines were dilated with inflammatory exudates and characterized by mucosal necrosis indicating Clostridium perfringens type A to be the major causative types for necrotic enteritis of broilers in Kashmir, India with significant role of alpha toxin.


Keywords: Clostridium perfringens, multiplex PCR, Toxin typing.
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How to cite this article:

Nyrah, Q., S.A. Wani, N. Nazir, S. Rasool, Q. Beigh, Z.A. Kashoo, I. Hussain, Sabia Qureshi and Ali, R. 2017. Clostridium perfringens Type A from Broiler Chicken with Necrotic Enteritis in Kashmir Valley, India.Int.J.Curr.Microbiol.App.Sci. 6(12): 2443-2453. doi: https://doi.org/10.20546/ijcmas.2017.612.283