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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2174-2183
DOI: https://doi.org/10.20546/ijcmas.2017.612.249


Identification of Pathogenic Leptospira spp. Carrier Goats Using Polymerase Chain Reaction (PCR)
Priti D. Vihol1*, Jatin H. Patel2, Jignesh M. Patel1, Vijendra S. Dabas3, Irshadullakhan H. Kalyani4, Chandrakant F. Chaudhari5 and Ashish C. Patel6
1Department of Veterinary Pathology
2Department of Veterinary Pharmacology and Toxicology
3Department of Surgery and Radiology
4Department of Veterinary Microbiology
5Department of Animal Reproduction Gynecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Navsari Agricultural University,Eru cross road, Navsari-396 450, Gujarat, India
6Department of Animal Genetics and Breeding, College of Veterinary Sciences and A.H. Anand Agricultural University, Anand- 388001, Gujarat, India
*Corresponding author
Abstract:

Leptospirosis, an important zoonotic disease with worldwide distribution, is emerging as public health problem in south Gujarat, India where it is endemic. In infected animals, persistent colonization of leptospires in renal tubules is not uncommon and hence chronically infected hosts shed leptospires in urine and contaminate the environment. A study was undertaken to identify leptospiral carrier goats in south Gujarat using urine polymerase chain reaction. A total of 292 urine and sera samples each were collected randomly from goats reared in different villages of south Gujarat region and studied using polymerase chain reaction (PCR) and microscopic agglutination test (MAT), respectively. In MAT, seroreactivity was noted in 61 (20.89 %) goats while leptospiral DNA was detected in 42 (14.38 %) goats using PCR. There was low concordance between two tests. To the best of author’s knowledge, it is the first study indicating use of PCR in detection of carrier goats from south Gujarat, India. Present findings indicate existence of carrier status of leptospirosis among goats in this area. In conclusion, leptospiral DNA was detected from urine of goats using PCR hence use of PCR may be considered as a valuable tool for identification of carrier goats. Present results also necessitate effective control measures in south Gujarat.


Keywords: Carrier, DNA, PCR, MAT, Leptospirosis.
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How to cite this article:

Priti D. Vihol, Jatin H. Patel, Jignesh M. Patel, Vijendra S. Dabas, Irshadullakhan H. Kalyani, Chandrakant F. Chaudhari and Ashish C. Patel. 2017. Identification of Pathogenic Leptospira spp. Carrier Goats Using Polymerase Chain Reaction (PCR).Int.J.Curr.Microbiol.App.Sci. 6(12): 2174-2183. doi: https://doi.org/10.20546/ijcmas.2017.612.249