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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2125-2133
DOI: https://doi.org/10.20546/ijcmas.2017.612.243


AmpC β-lactamase Production in Pseudomonas aeruginosa: A Threat
Arti Agrawal1, Namita Srivastava1, Dharmendra Kumar2*, Rashmi2 and Neha Bhati2
1Department of Microbiology, Sarojini Naidu Medical College, Agra (Uttar Pradesh), India
2Department of Microbiology, Chhatrapati Shahujii Maharaj University,
Kanpur (Uttar Pradesh), India
*Corresponding author
Abstract:

Pseudomonas aeruginosa is responsible for the devastating nosocomial infections. Class C of cephalosporinase (AmpC β-lactamases) is important cause of multiple β-lactam resistance in P. aeruginosa. The aim of this study is to detect the AmpC β-lactamase production and Antimicrobial susceptibility pattern in Pseudomonas aeruginosa isolated from clinical samples in Tertiary health care Hospital. This study was conducted over a period of January (2016) to July (2016) in department of Microbiology, Sarojini Naidu Medical College, Agra (Uttar Pradesh), India. The organisms were identified on the basis of their culture characteristics and battery of Biochemical tests. Antimicrobial susceptibility of all the isolates was performed by the Kirby- Bauer disc-diffusion method. AmpC β-lactamase production was determined by the modified 3D test. 159 isolates of P. aeruginosa were obtained from different specimens. Maximum numbers of P. aeruginosa isolates were resistant to Piperacillin followed by Gentamycin, Cefepime and Ceftazidime. While most isolates were sensitive to Polymyxin-B, Colistin, Amikacin, Imipenem, and Meropenem. A high prevalence of AmpC β-lactamases production was observed amongst the isolates of P. aeruginosa as compared to other studies. Isolates of AmpC β-lactamases producing Pseudomonas aeruginosa have been continuously reported globally with some disparity in the rates of resistance.


Keywords: Pseudomonas aeruginosa, AmpC β-lactamases, AmpC β-lactamase Screening and Confirmatory 3D test.
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How to cite this article:

Arti Agrawal, Namita Srivastava, Dharmendra Kumar, Rashmi and Neha Bhati. 2017. AmpC β-lactamase Production in Pseudomonas aeruginosa: A Threat.Int.J.Curr.Microbiol.App.Sci. 6(12): 2125-2133. doi: https://doi.org/10.20546/ijcmas.2017.612.243