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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2099-2107
DOI: https://doi.org/10.20546/ijcmas.2017.612.240


Bacterial Population Associated with Fruits and Vegetables and its Treatment using Antimicrobial Rinsing
Amandeep Kaur*, Anchal Sood, Sarbjit Kaur and Priyanka Bhowate
Dolphin College of Life Sciences, Chunni Kalan Dist. Fatehgarh Sahib, Punjab, India
*Corresponding author
Abstract:

Fruits and vegetables are one of the essential diets of human being, which they are consumed widely. But, they are contaminated with different bacteria that may cause food borne illness. Bacteriological survey of 20 samples of fresh vegetables and fruits were done to study the density of microorganisms by standard plate count (SPC) method. The colony forming units per gram (cfu/g) of the vegetables were much less (7.2x105 to 30.4x105) in local market sample as compared to super market sample (12.0x105 to 20.6x105) and field sample (8.0x105 to 18.0x105). But in case of fruits higher cfu were obtained for the field sample (8x105) as compared to local market sample (4x105 to 5.5x105) and super market sample (2.5x105). Of the various pathogens identified from the surface of these samples Escherichia coli, Staphylococcus aureus, Bacillus cerous were found to be the pre-dominant species in most of the samples. It was observed that the Bacillus, Enterobacter and Staphylococcus were the dominant genera in vegetables. On the basis of antimicrobial rinsing of carrot, which was carrying the highest bacterial contamination, five different anti-microbial agents (Hydrogen peroxide, Sodium chloride, Calcium chloride, Sodium citrate and Ethanol) decreased the total number of microbial organisms’ load of the carrot by 40-80 per cent. Hydrogen peroxide was, however, found to be the most effective, as rinsing treatment with it showed more reduction in total number of bacterial organisms in 80 per cent of samples. Hydrogen peroxide can thus be used as rinsing agent to reduce this type of superficial bacterial contamination so as to improve the quality of fresh vegetables and fruits for human consumption. The microflora of fruits and vegetables are one of the great concerns in our society as they can be of great risk for human health. Proper handling, hygiene, transportation and proper storage is necessary to avoid microbial food spoilage and related health risks.


Keywords: Fruits, Vegetables, food pathogens, Microbiological load, Bacteriological analysis.
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How to cite this article:

Amandeep Kaur, Anchal Sood, Sarbjit Kaur and Priyanka Bhowate. 2017. Bacterial Population Associated with Fruits and Vegetables and its Treatment using Antimicrobial Rinsing.Int.J.Curr.Microbiol.App.Sci. 6(12): 2099-2107. doi: https://doi.org/10.20546/ijcmas.2017.612.240