The present study was aimed to analyse the nutritional composition and antibacterial activity of selected green macroalgae from Andaman Islands. Proximate composition was estimated for the selected four green macroalgae species namely, Halimeda macroloba, Halimeda tuna, Enteromorpha sp. and Acetabularia acetabulum. Methanol and Dimethyl Sulphoxide (DMSO) dissolved extracts of these macroalgae were tested against human and fish pathogens such as Staphylococcus aureus, Aeromonas hydrophila, Escherichia coli and Streptococcus pneumoniae. The results revealed that maximum protein content was observed in Enteromorpha sp. (16.56 ± 2.45) while minimum was observed in A. acetabulum (8.123 ± 0.89). All the analysed samples were high in ash content which ranged from 18.56 ± 0.12 to 33.67 ± 0.23. Among all the analysed macroalgae species, Enteromorpha sp. showed highest zone of inhibition against all the pathogens tested and S. aureus showed sensitivity to the methanolic extracts of all the four macroalgae species. Considerable level of protein, dietary fibre and high ash content were observed in all the analysed macroalgae species. The methanolic extract of Enteromorpha sp. was found active against all the tested pathogens. Thus macro algae can serve as a source of food protein and an ingredient of high nutritional value in human and animal diet and can even be used against pathogenic infection in human and animal treatment.

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