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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 1723-1728
DOI: https://doi.org/10.20546/ijcmas.2017.612.195


Polymerase Chain Reaction in the Diagnosis of Spontaneous Leptospirosis in Bovines
J.M. Patel1*, P.D. Vihol1, M.C. Prasad1, J.H. Patel2, J.K. Raval3 and I.H. Kalyani4
1Department of Veterinary Pathology
2Department of Veterinary Pharmacology and Toxicology
3Department of Teaching Veterinary Clinical Complex
4Department of Veterinary Microbiology, Vanbandhu College of Veterinary Science and Animal husbandry, Navsari Agricultural University, Navsari, India
*Corresponding author
Abstract:

Accurate and prompt diagnosis of leptospiral infection in bovine is difficult due to limitation of current procedures. The present study describes the application of polymerase chain reaction (PCR) for detection of leptospiral deoxyribonucleic acid (DNA) in blood and urine samples in the spontaneous cases of bovine leptospirosis. In most of studies leptospires DNA detected by PCR in experimentally induced infection so in present study trying to evaluate this assay in naturally infected cattle and buffalo. A total of 120 blood and 304 urine samples were collected from cattle and buffaloes. These samples were subjected to PCR using two sets of primers i.e. G1/G2 and LipL32 and it revealed that 9/120 blood and 33/304 urine samples were found to be positive. The results suggested that PCR could be an excellent approach for diagnosis of Leptospirosis in field samples also. The specificity of primers was checked by using reference pathogenic serovar of Leptospira.


Keywords: Diagnosis, G1/G2, LipL32, PCR, Leptospirosis.
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How to cite this article:

Patel, J.M., P.D. Vihol, M.C. Prasad, J.H. Patel, J.K. Raval and Kalyani, I.H. 2017. Polymerase Chain Reaction in the Diagnosis of Spontaneous Leptospirosis in Bovines.Int.J.Curr.Microbiol.App.Sci. 6(12): 1723-1728. doi: https://doi.org/10.20546/ijcmas.2017.612.195