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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 1499-1510
DOI: https://doi.org/10.20546/ijcmas.2017.612.167


Optimization of Fermentative Production of Keratinase by Bacillus subtilis Strain S1 in Submerged State Fermentation Using Feather Waste
Sandeep Singh1*, Harison Masih1, G. Ebenezer Jeyakumar2, Rubina Lawrence1 and P.W. Ramteke3
1Department of Microbiology and Fermentation Technology, SHUATS, Allahabad, India
2Department of Clinical Laboratory Science, SHUATS, Allahabad, India
3Department of Biological Sciences SHUATS, Allahabad, India
*Corresponding author
Abstract:

Keratinases are well-recognized enzymes with the singular power of taking down the recalcitrant structural proteins such as keratin. Their prospective in bio-waste management of feather waste has been well recognized since long. In present scenario, they have acquired importance in various other industrial applications. The present study mainly focused on the isolation of keratin degrading bacteria and its use in optimization of culture conditions to maximize the keratinase production. The isolated strain was identified as Bacillus subtilis strainS1 according to morphological and biochemical characteristics followed by 16 S rRNA sequencing (accession no: LC054177). The influence of cultivation temperature and initial pH of the medium on keratinase production revealed the optimal values of the temperature and pH as 40 °C and 7, respectively. Maximum keratinolytic activity was observed at 72 h after incubation. Optimized value for inoculum size and substrate concentration was found to be 5% and 1% respectively, 150 rpm found to be the optimum agitation level. The best additional nitrogen source was Beaf extract and all the additional carbon sources showed a negative effect on keratinase production. These results indicate that this bacterial strain shows a high potential for keratinase production in submerged-state fermentation, and use of feather waste as the substrate can be implemented for keratinous solid waste management.


Keywords: Bacillus subtilis, Keratinase production, Submerged-state fermentation, Feather waste.
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How to cite this article:

Sandeep Singh, Harison Masih, G. Ebenezer Jeyakumar, Rubina Lawrence and Ramteke, P.W. 2017. Optimization of Fermentative Production of Keratinase by Bacillus subtilis Strain S1 in Submerged State Fermentation Using Feather Waste.Int.J.Curr.Microbiol.App.Sci. 6(12): 1499-1510. doi: https://doi.org/10.20546/ijcmas.2017.612.167