This study was carried out on semen of three mature Gir cattle and three Surti buffalo bulls. The semen ejaculates (9/bull, total 54) collected in AV were evaluated for sperm motility, viability, morphology, acrosomal integrity, plasma membrane integrity (HOST) and enzyme GOT-GPT leakage pre- and post-freezing in TFYG diluent including post-thaw longevity till 3 h of incubation and their interrelationships. Most of the sperm parameters studied at initial, pre-freeze (PF) and post-thaw (PT) stages were highly significantly and positively interrelated in both the species (0.67 to 0.99). In Gir bulls, significant (P<0.01) correlations were observed for sperm concentration with PF and PT GOT (0.59, 0.57) as well as GPT (0.48, 0.48), and with PT HOS reactive sperm (-0.43); individual sperm motility in fresh semen with PF sperm motility and live sperm (0.94, 0.49) as well as PT motility at 0 and 60 min (0.46, 0.45) of incubation; initial HOS reactive sperm with PT abnormal sperm (-0.58); initial live sperm with PT motility and longevity (0.55, 0.54); pre-freeze motility with PF & PT live sperm (0.54, 0.55) and PT motility at 0 and 60 min (0.48, 0.45); pre-freeze HOS reactive sperm with PF abnormal sperm (-0.56); pre-freeze live sperm with PF intact acrosome (0.51); pre-freeze GOT with PT HOS reactive sperm (-0.45); post-thaw motility with PT live sperm (0.44) and longevity after 60 and 120 min of incubation (0.90, 0.81); post-thaw HOS reactive sperm with PT GOT (-0.47) and incubation motility (0.43); and post-thaw live sperm with PT intact acrosome (0.44). In Surti bulls, the initial sperm motility had significant (P<0.01) correlations with PF HOS reactive sperm (0.58) as well as PF & PT intact acrosome (0.55, 0.51) and GOT (0.39, 0.44); initial HOS reactive sperm with initial and PF live sperm (0.48, 0.42) and GOT (-0.42, -0.41); initial live sperm with HOS reactive sperm (-0.44); initial intact acrosome with PF motility (0.43) and live sperm (0.51); initial GOT with initial, PF and PT GPT (0.72, 0.53, 0.54) and with PT incubation motility (0.38); pre-freeze sperm motility with PF HOS reactive sperm and intact acrosome (0.64, 0.56) and with PT intact acrosome (0.46); pre-freeze HOS reactive sperm with PF & PT live sperm (0.57, 0.69) and intact acrosome (0.54, 0.66); pre-freeze live sperm with PF & PT intact acrosome (0.69, 0.70); pre-freeze intact acrosome with PT live sperm (0.46); pre-freeze GOT with PT motility (0.39); post-thaw motility with PT GOT (0.43) and PT longevity after 60 and 120 min (0.91, 0.65); post-thaw live sperm with PT intact acrosome (0.62) and incubation motility (0.54); and post-freeze GOT with PT GPT (0.60) and PT longevity of sperm (0.47).

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