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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 553-559
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.056


Phenotypic Differentiation of Metallo beta Lactamases and Class A KPC Carbapenemases in Enterobacteriaceae Isolates
Gomty Mahajan, Shashi Chopra, Jaspal Kaur, Sheevani and Kailash Chand
Department of Microbiology, Punjab institute of Medical Sciences, Jalandhar, India
*Corresponding author
Abstract:

Carbapenemase producers are increasingly reported worldwide in Enterobacteriaceae. Carbapenems are usually the choice of antimicrobials in infections caused by Enterobacteriaceae. Resistance to carbapenems is mostly due to production of enzymes - Carbapenemases, which are divided into Ambler Classes A, B and D. Phenotypic detection and differentiation of carbapenemases in Enterobacteriaceae is of great help to the patients as well as clinicians for proper infection control and appropriate patient management. The aim of the present study was to evaluate the performance of modified Hodge test and combined disc test for detection and differentiation of carbapenemase production. A study was conducted on 41 Carbapenem Resistant Enterobacteriaceae (CRE) strains which were isolated from various clinical samples over a period of one year (September 2013-Agust 2014). All the isolates were identified by standard microbiological procedures and antibiotic sensitivity was performed according to CLSI guidelines. Isolates were tested for carbapenemase production by modified Hodge test (MHT) and combined disc test. Phenotypic confirmatory test was done by using discs of Meropenem alone and those with phenyl boronic acid (PBA) or Ethylenediamnetetra acetic acid (EDTA) or both, for the detection of carbapenemase production and differentiation of KPC and MBL enzymes. Out of these 482strains, 41 (8.5%) strains were resistant to carbapenems. Among the 41 carbapenem resistant isolates, 29 isolates were positive and 12 isolates were negative by MHT. Of these 41 carbapenem resistant clinical isolates of Enterobacteriaceae, 29 isolates were producing KPC, MBL and KPC+MBL enzymes. Rest were negative for KPC and MBL by combined disc test. We conclude that the need of the hour is simple, rapid and cost effective tests which will be able to identify and distinguish resistant and can be easily incorporated in routine microbiology lab testing


Keywords: Class A KPC, Metallo beta lactamases, PBA, EDTA, Carbapenem Resistant Enterobacteriace ae (CRE)
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How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 553-559. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.056