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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 1369-1380
DOI: https://doi.org/10.20546/ijcmas.2017.612.153


Isolation and Antibiogram of Nonfermentative Gram Negative Bacilli in various Clinical Specimens in a Tertiary Care Hospital, Jaipur, India
Jitendra1, Shiv Kumar1*, Sheetal Sharma2, Jogender1 and Suman Rishi1
1Department of Microbiology, NIMS Medical College, Jaipur, Rajasthan, India
2Department of Microbiology, Pacific Medical College, Udaipur, Rajasthan, India
*Corresponding author
Abstract:

Non Fermentative Gram Negative Bacilli were regarded to be Nonpathogenic and Contaminants in the past. Recently these bacilli became impending pathogenic with the ability to show resistance for commonly used antibiotics. These organisms isolated from clinical specimens with increasing frequency. So this study planned to isolate and antibiogram of Non-fermentative gram negative bacilli in various clinical specimens at tertiary care hospital at Jaipur. The present study was carried out on 49 of strains of Nonfermenters isolated from 397 non repetitive clinical samples like Pus, Wound discharge, Blood culture, Urine, Sputum, Ear discharge, Body fluid, ET Secretions and CSF etc. from all age’s group. Conventional bacteriological methods were used for identification non fermenting gram negative bacilli and susceptibility testing was performed with the help of the Modified Kirby-Bauer disc diffusion method. This study comprises of 49 isolates of Non Fermentative Gram Negative Bacilli from total of 397 clinical specimens, collected from in- patients admitted to various departments during a period of 6 months. Pseudomonas aeruginosa was the most common isolate (57.14%) while Acinetobacter baumannii (24.48%) was the second most common isolated followed by Pseudomonas fluorescens (14.28%) and Acinetobacter lwoffii (4.08%). Among all clinical samples Pus yield maximum isolates of NFGNB i.e. 15(30.61%) followed by same account of Urine 8(16.32%) and Sputum 8(16.32%). Most sensitive drug against NFGNB were Polymyxin-B (100%) and Colistin (100%) followed by Imipenem (81.63%), Amikacin (71.42%). Pseudomonas aeruginosa and Acinetobacter baumannii have emerged as the most common Nonfermentative gram negative bacilli causing human infections. Alarming resistance rate was found towards commonly used antibiotics. Polymyxin B and Colistin showed excellent susceptibility against such bacteria followed by Imipenem and amikacin.


Keywords: Nonfermenters, Antibiogram, Modified Kirby-Bauer disc diffusion method, Nosocomial infections, Healthcare associated infections, Polymyxin-B, Colistin.
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How to cite this article:

Jitendra, Shiv Kumar, Sheetal Sharma, Jogender and Suman Rishi. 2017. Isolation and Antibiogram of Nonfermentative Gram Negative Bacilli in various Clinical Specimens in a Tertiary Care Hospital, Jaipur’’Int.J.Curr.Microbiol.App.Sci. 6(12): 1369-1380. doi: https://doi.org/10.20546/ijcmas.2017.612.153