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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 1349-1368
DOI: https://doi.org/10.20546/ijcmas.2017.612.152


Pseudomonas and Proteus: Partial Purification and Characterization of Antibacterial Protein: A Review
N.T. Eshwari* and M. Kannahi
Department of Microbiology, Sengamala Thayaar Educational Trust Women`s College, Sundarakkotai, Mannargudi – 614001, Tamil Nadu, India
*Corresponding author
Abstract:

Marine water samples were collected from a coastal region of Pushpavanam, Nagapattinam District, Tamil Nadu, India. Marine Pseudomonas and Proteus were isolated and identified according to morphology, biochemical test and selective complex marine growth medium. The identified marine organisms were maintained with LB broth. The cultures were harvested by cooling centrifugation at 9,000x g for 20 min. This suspension was incubated at 28ºC for 24 hours after incubation the cells removed by centrifugation method. The supernatant was filter sterilized and antibacterial activity was zone of inhibition performed by measuring overlay assay technique and drop test assay method. Samples were suspended in sucrose – dye – solution and identification of antibacterial protein band resolved by polyacrylamide gel electrophoresis. The gel was washed with distilled water. The protein fraction was identified by the drop test. All the proteins were assayed with a bicinchoninic acid protein kit. The protein fraction elution was done with phosphate buffer solution. The protein fraction was stored at 20ºC after freeze – drying. The antibacterial protein was detected within SDS-PAGE treatment with Coomassie brilliant blue. The concentration level of antibacterial protein is necessary to result in cell death and to inhibit the growth of marine microorganisms in suitable medium. Bacterial cells were inoculated at time zero and the bacterial growth rates and cell viability were measured by colony forming unit.


Keywords: Drop test assay, Protein, Antibacterial activity, Colony forming unit, Gel electrophoresis.
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How to cite this article:

Eshwari, N.T. and Kannahi, M. 2017. Pseudomonas and Proteus: Partial Purification and Characterization of Antibacterial Protein: A Review.Int.J.Curr.Microbiol.App.Sci. 6(12): 1349-1368. doi: https://doi.org/10.20546/ijcmas.2017.612.152