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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 1215-1231
DOI: https://doi.org/10.20546/ijcmas.2017.612.138


Characterization and Optimization of Lipase Production from Soil Microorganism (Serratia marcescens)
E. Nwachukwu1, E.N. Ejike3*, B.U. Ejike2, E.O. Onyeanula1, R.O. Chikezie-Abba1, N.A. Okorocha4 and U.E. Onukaogu3
1Department of Microbiology
2Department of Zoology and Environmental Biology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
3Department of Biology/Microbiology Abia State Polytechnic, Aba Abia State Nigeria
4Abia State Teaching Hospital, Aba, Abia State Nigeria
*Corresponding author
Abstract:

Lipase production by lipolytic bacteria (Serratia marcescens), isolated from soil in Ohuhu Community in Umuahia North Local Government Area of Abia State, was investigated using a basal medium containing KH2PO4, Na2SO4, MgSO4.7H2O, Yeast extract, K2HPO4, Peptone, Glucose and Olive oil in a shake flask culture at 370C for 48hours. The study of different carbon sources on lipase production revealed that glucose yielded higher lipase production of 7.6Uml-1 than sucrose (6.2Uml-1), fructose (4.9Uml-1), starch (3.6Uml-1), lactose (2.4Uml-1) and maltose (1.4Uml-1). Subsequently, 2.5% concentration of glucose yielded higher lipase activity than the other concentrations (0.5%-4%) of glucose tested. Screening for the best nitrogen source revealed that peptone gave the highest activity of 28.5Uml-1, others are tryptone (25.5Uml-1), yeast extract (20.0Uml-1) and meat extract (22.4Uml-1), while NH4Cl showed the least activity of 7.0Uml-1. Also, 2.5% concentration of peptone gave the highest activity compared with other concentrations (0.5-4.5%) tested. Among the natural oils, olive oil gave the highest lipase activity of 25.2Uml-1 compared with other oils and 0.8% concentration of olive oil was found to increase lipase production by 36.2Uml-1. Other lipid sources observed were palm oil (20.5Uml-1), groundnut oil (19.5Uml-1), soy oil (16.4Uml-1) and crude oil (14.8Uml-1). The most suitable source of metal ion was MgSO4 (29.5Uml-1) followed by MnSO4 (27.9Uml-1), KCl had the least activity of 13.9Uml-1. Organic solvents on lipase activity revealed that methanol yielded higher lipase activity of 42.7Uml-1 than ethanol (30.5Uml-1), isopropanol (15.6Uml-1) and acetone (5.2Uml-1). The results promises high production of lipase with an inexpensive and simple medium containing palm oil, peptone, and magnesium sulfate, at pH 6.0 and 150 rpm for 48hrs (2days). This could be employed in the enzyme industry for commercial purpose.


Keywords: Lipase, Serratia marcescens, Lipolytic bacteria, Charaterization and Optimization.
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How to cite this article:

Nwachukwu, E., E.N. Ejike, B.U. Ejike, E.O. Onyeanula, R.O. Chikezie-Abba, N.A. Okorocha and Onukaogu, U.E. 2017. Characterization and Optimization of Lipase Production from Soil Microorganism (Serratia marcescens).Int.J.Curr.Microbiol.App.Sci. 6(12): 1215-1231. doi: https://doi.org/10.20546/ijcmas.2017.612.138