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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 905-910
DOI: https://doi.org/10.20546/ijcmas.2017.612.098


A Simple Chromatographic (HPLC) Method for the Determination of β- Carotene Contents in Inbred Maize Genotypes
Anubhuti Sharma1, Nisha Malik2, Vijaya Laxmi Tripathi2, Priti Gupta2 and Rashmi2*
1ICAR-DRMR, Bharatpur, India
2Banasthali Vidyapith, India
*Corresponding author
Abstract:

The paper presents a method for determination of β-carotene concentration from 50 maize genotypes carotenoidic extracts using the high performance liquid chromatography (HPLC). The analysed genotypes were cultivated at the Vivekanand Parvatiya Krishi Anusandhan Sansthan, Almora (Uttarakhand). In view of a more complete carotenoidic pigments liberation from the raw material, it was used an improved carotenoids extraction technique. In this purpose the maize flour was moisten with distilled water and then treated with ethanol and let at rest 50 minutes for starch hydrolysis and advanced liberation of the carotenoids from plants cells. Carotenoidic pigments extraction was achieved with an organic solvents mixture of Petroleum ether: Diethyl etherto colorless. β-Carotene was identified in all the maize genotypes. Selected three analyzed maize lines (V391, V351 and V345) presents higher β–carotene content, compared with the literature data concerning β–carotene content in other maize hybrids.


Keywords: Chromatographic, Determination, Maize.
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How to cite this article:

Anubhuti Sharma, Nisha Malik, Vijaya Laxmi Tripathi, Priti Gupta and Rashmi. 2017. A Simple Chromatographic (HPLC) Method for the Determination of β- Carotene Contents in Inbred Maize Genotypes.Int.J.Curr.Microbiol.App.Sci. 6(12): 905-910. doi: https://doi.org/10.20546/ijcmas.2017.612.098