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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 719-728
DOI: https://doi.org/10.20546/ijcmas.2017.612.075


VIS-NIR Reflectance Spectroscopy for Assessment of Soil Microbiological Properties
Bhabani Prasad Mondal1*, Bharpoor S. Sekhon1, Sandeep Sharma1, Manjeet Singh2, Rabi N. Sahoo3, Arijit Barman4, Yagani Sinha5, Arghya Chattopadhyay6 and Koushik Banerjee3
1Department of Soil Science
2Department of Farm Machinery and Power Engineering,Punjab Agricultural University, Ludhiana, Punjab-141004, India
3Division of Agricultural Physics, Indian Agricultural Research Institute,
New Delhi-110012, India
4Division of Soil and Crop Management, ICAR-Central Soil Salinity Research Institute,Karnal, Hariyana-132001, India
5Department of Soil Science and Agricultural Chemistry, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharastra-444104, India
6Department of Soil Science and Agricultural Chemistry,Banaras Hindu University, Varanasi-221005, India
*Corresponding author
Abstract:

Soil microbes play an important role in major processes of soil system. Rapid, reliable and cost effective assessment of soil microbiological properties is highly essential for monitoring soil health. Traditional chemical-based soil analysis is laborious, hazardous and time consuming. Visible near-infrared (VIS-NIR) reflectance spectroscopy can be a viable alternative to ensure rapid and environmentally safe soil test results. With this objective, two key soil microbiological parameters microbial biomass carbon (MBC) and dehydrogenase activity (DHA) were examined for their amenability to diffuse reflectance spectroscopy assessment. Geo referenced surface soil (0-15cm) samples (n=170) were collected from different fields for spectral analysis. Partial least square regression (PLSR) technique was employed for developing spectral models. The different model evaluation indices like coefficient of determination (R2), root mean square error of prediction (RMSE) and ratio of performance deviation (RPD) were used to evaluate the predictive performance of model. The RPD values for MBC and DHA were 1.15 and 1.13. Poor predictive performance of our model as opposed to substantial evidence in literature can be ascribed to range of factors like limited variability in spectra, relatively smaller sample size, use of entire spectra instead of selective regions and use of samples collected under natural farm environment as opposed to those generated under controlled conditions.


Keywords: Dehydrogenase, Land use, Microbial biomass carbon, Reflectance spectroscopy, VIS-NIR models.
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How to cite this article:

Bhabani Prasad Mondal, Bharpoor S. Sekhon, Sandeep Sharma, Manjeet Singh, Rabi N. Sahoo, Arijit Barman, Yagani Sinha, Arghya Chattopadhyay and Koushik Banerjee. 2017. VIS-NIR Reflectance Spectroscopy for Assessment of Soil Microbiological Properties.Int.J.Curr.Microbiol.App.Sci. 6(12): 719-728. doi: https://doi.org/10.20546/ijcmas.2017.612.075