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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 711-718
DOI: https://doi.org/10.20546/ijcmas.2017.612.074


Detection of Methyl Parathion Residues in Vegetables by Using Chromogenic Reagent
S. Easwari* and R. Jayakumar
Department of Soil Science and Agricultural Chemistry, TNAU, Coimbatore-3, Tamil Nadu, India
*Corresponding author
Abstract:

In the present investigation the organophosphorus insecticides viz., chlorpyrifos, dichlorvos, malathion, methyl parathion, monocrotophos, phosphamidon, profenofos, quinalphos and triazophos insecticides were assessed with different chromogenic reagents. Initial screening of the chromogenic reagents to detect organophosphorus insecticides was done through paper strip method. In paper strip method, development of yellow colour was observed for methyl parathion using sodium hydroxide as chromogenic reagent. Phosphamidon and quinalphos were detected by developing brown and white colour using silver nitrate and sodium hydroxide. The darkness of colour on the spot was increased with increased concentrations. The fortification studies were conducted to determine the methyl parathion, phosphamidon and quinalphos through paper strip method. The results reported that using sodium hydroxide yellow colour was appeared in methyl parathion residual extract. Where as in phosphamidon and quinalphos, the residual extract when treated with silver nitrate the colour obtained was not comparable with standards. Validation was done for methyl parathion using standards and residual extracts and the results were comparable with standards as well fortified residual extracts. From the above experimental results, a test kit was developed for methyl parathion residues detection. The developed test kit was used to determine the pesticide residues present in market vegetable samples.


Keywords: Detection, Methyl parathion, Pesticide residues, Vegetables, Chromogenic reagents.
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How to cite this article:

Easwari, S. and Jayakumar, R. 2017. Detection of Methyl Parathion Residues in Vegetables by Using Chromogenic Reagent.Int.J.Curr.Microbiol.App.Sci. 6(12): 711-718. doi: https://doi.org/10.20546/ijcmas.2017.612.074