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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 574-584
DOI: https://doi.org/10.20546/ijcmas.2017.612.070


In vitro Inhibition of Pectolytic Enzymes of Erwinia chrysanthemi by Combined Application of Pseudomonas fluorescens and Bacillus subtilis on Aloe vera
M. Syamala* and C. Ciba
Department of Plant Pathology and Department of Horticulture, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India
*Corresponding author
Abstract:

In an attempt to develop biocontrol system for management of soft rot in Aloe vera, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their antagonistic activity against Erwinia chrysanthemi in vitro. Soft rot diseases caused by the Erwinia chrysanthemi lead to significant yield losses of crops. Experiments were conducted on the effect of culture filtrates of combined application of (Pf32 + Pf 45 +Bs5) on the in vitro inhibition of pectinolytic enzymes of Erwinia chrysanthemi. The activity of pectate lyase activity, pectin methyl esterase, polygalacturonase, pectin trans eliminase activity produced by Erwinia chrysanthemi was higher, when compared to control. Maximum inhibition of above pectinolytic enzymes (PME, PG and PTE) was shown by Pseudomonas fluorescence treatment was followed Bacillus subtilis. Of all the treatments, Pseudomonas fluorescence treatment showed higher rate of inhibition of Pectinolytic enzymes of Erwinia chrysanthemi. This study indicates that combined application of (Pf32 + Pf 45 +Bs5) is the best biocontrol agent in the inhibition of Erwinia chrysanthemi causing soft rot of Aloe vera. The enzyme activity of inoculated aloe plants increased from the two day till the six DAI and slowly declined thereafter in all the 15 isolates.


Keywords: Pseudomonas fluorescens, Bacillus subtilis.
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How to cite this article:

Syamala, M. and Ciba, C. 2017. In vitro Inhibition of Pectolytic Enzymes of Erwinia chrysanthemi by Combined Application of Pseudomonas fluorescens and Bacillus subtilis on Aloe veraInt.J.Curr.Microbiol.App.Sci. 6(12): 574-584. doi: https://doi.org/10.20546/ijcmas.2017.612.070