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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 543-550
DOI: https://doi.org/10.20546/ijcmas.2017.612.066


Genetic Diversity of Maize (Zea mays L.) Inbred Lines Revealed by Simple Sequence Repeat Markers
Savita Sharma1*, D.P. Mishra1, Bhupender Kumar2, Harpreet Kaur2 and H.R. Jewlia2
1Shri Venkateshwara University, UP, India
2ICAR-Indian Institute of Maize Research, New Delhi-110012, India
*Corresponding author
Abstract:

A set of 33 maize inbred lines were analyzed using 40 Simple Sequence Repeat (SSR) markers. Out of 40 SSR markers, 25 markers were found reproducible in which 18 were found polymorphic and 7 monomorphic. Total 40 alleles ranges from 2 to 3 with mean of 2.22 alleles per loucs were identified among 33 lines using18 polymorphic SSR markers. The average polymorphism Information Content (PIC) was 0.36 with range of 0.1 to 0.56. Unweighted Paired Group Method using Arithmetic Averages (UPGMA) cluster analysis grouped the genotypes into two major clusters. Cluster I was the biggest one with 25 genotypes which was further sub-divided into two sub-clusters with 21 and 4 genotypes, respectively. The major cluster II contained 9 genotypes in two sub clusters each with 7 and 2 genotypes. The identified clusters may find useful to attempt crossing programme in maize breeding programme. In this study, we found SSR as a good complementing tool along with morphological markers for maize genetic characterization.


Keywords: Zea mays, Simple sequence repeat, Genetic diversity, Maize clusters.
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How to cite this article:

Savita Sharma, D.P. Mishra, Bhupender Kumar, Harpreet Kaur and Jewlia, H.R. 2017. Genetic Diversity of Maize (Zea mays L.) Inbred Lines Revealed by Simple Sequence Repeat Markers.Int.J.Curr.Microbiol.App.Sci. 6(12): 543-550. doi: https://doi.org/10.20546/ijcmas.2017.612.066