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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:12, December, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 500-507
DOI: https://doi.org/10.20546/ijcmas.2017.612.061


Molecular Diversity Study in CMS-A, Maintainer-B and Restorer-R Lines in Pigeon Pea [Cajanus cajan (L.) Millsp.]
Mayur Gadekar1*, Rohit Jadhav2, Milind Meshram2, Shreekant Amarshettiwar2, Ashok Patil2 and Ravindra Nandanwar2
1Nimbkar Agricultural Research Institute, Phaltan, Maharashtra, India
2Department of Agricultural Botany, Post Graduate Institute, Dr. P.D.K.V.,Akola, Maharashtra, India
*Corresponding author
Abstract:

Twenty eight lines including 8 A lines, 8 B-lines and 12 R-lines newly developed at station were characterized by with 21 SSR primers. A total number of alleles detected by the aforesaid primers were 63 with maximum and minimum frequency of 5 and 2, respectively. The maximum and minimum PIC Values were found to be 0.994 and 0.138 for the primers CCB-3 and CCB-5 respectively. Average genetic similarity coefficient from all possible combinations was found to be 0.563. Among the 21 primers, 9 were found polymorphic for 28 genotypes. Genetic similarity coefficient for most of number of pairs was in the range of 0.86 to 1.00, indicating diversity among the chosen genotypes. All the 28 genetic lines could be easily distinguished from each other based on the selected markers and thus were effective for varietal identification and diversity analysis of pigeonpea genotypes. A dendogram based on loci profile showed good agreement with their cultivars pedigree information.


Keywords: Molecular diversity, Pigeon pea.

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How to cite this article:

Mayur Gadekar, Rohit Jadhav, Milind Meshram, Shreekant Amarshettiwar, Ashok Patil and Ravindra Nandanwar. 2017. Molecular Diversity Study in CMS-A, Maintainer-B and Restorer-R Lines in Pigeon Pea [Cajanus cajan (L.) Millsp.].Int.J.Curr.Microbiol.App.Sci. 6(12): 500-507. doi: https://doi.org/10.20546/ijcmas.2017.612.061
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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