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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 546-552
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.055


Obtaining of Lactobacillus delbrueckii Cold Sensitive Rif Mutants for Shelf Life Prolongation of Dairy Products
Alireza G. Goodarzi
SPC Armbiotechnology NAS, Yerevan, Armenia
*Corresponding author
Abstract:

The present study was aimed at using cold-sensitive (CS) starter as powerful alternative for shelf life prolongation of fermented dairy products. Utilized replica plating technique to select six cold-sensitive mutants of Lactobacillus delbrueckii MDC 9617 from about 400 rifampicin-resistant (Rif) mutants which losing ability to grow on LAPTg and ferment milk at minimal temperature. Whole CS mutants during growth at optimal temperaturebasically hold milk clotting rate, as well as the organoleptic properties of fermented dairy product. There are two CS Rif mutants CS-Rif3 and CS-Rif 8also containing highgrowth and milk fermentation rates property. Dairy Products which fermented by CS starters maintain the viable cells count, titratable acidity, texture and flavor at last for one month of refrigerator condition inmutuality to the native starter.Thereby, cold sensitive rif mutations can be used as an alternative solution for shelf life prolongation of dairy products at refrigerator temperatures also for technological characteristics ameliorate of starter cultures.


Keywords: Lactobacillus delbrueckii, Milk fermentation, Rifampicinresistant mutants, Shelf life
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How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 546-552. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.055