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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 434-440
DOI: https://doi.org/10.20546/ijcmas.2017.612.053


In Vitro Regeneration of Oat (Avena sativa L.) Using Different Explants
M.D. Salunke1, B.U. Rathod2* and N.R. Dattagonde1
1Department of Crop Science, Mahatma Gandhi College of Agricultural Biotechnology,
Nanded, Maharashtra, India
2Department of Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India
*Corresponding author
Abstract:

In present study efforts were made to improve the regeneration protocol in the oat cultivars JO-1 and OS-6. In mature embryo and immature embryo, callus induction was observed maximum on MS medium supplemented with 2,4-D (4.0mg/L and 6mg/L) respectively whereas, in case of embryogenic calli MS medium supplemented with 2,4-D (6.0 mg/L) was found to be best and in organogenesis MS medium supplemented with BAP (2-4mg/L) and NAA (0.1mg/L) was found to be better. In case of leaf base, callus induction was observed maximum on MS medium supplemented with 2,4-D (2.0 mg/L) whereas, in case of embryogenic calli MS medium supplemented with 2,4-D (2.0 mg/L) was found to be better and in organogenesis MS medium supplemented with BAP (2-4 mg/L) and NAA (0.1 mg/L) was found to be better. Among different genotypes JO-1 produced maximum number of induced calli, embryogenic calli as well as organogenic calli. While, genotype OS-6 performed moderately for initiation of calli.


Keywords: Oat, Regeneration, 2,4-D, BAP, IBA. NAA.
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How to cite this article:

Salunke, M.D., B.U. Rathod and Dattagonde, N.R. 2017. In Vitro Regeneration of Oat (Avena sativa L.) Using Different Explants.Int.J.Curr.Microbiol.App.Sci. 6(12): 434-440. doi: https://doi.org/10.20546/ijcmas.2017.612.053