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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 266-274
DOI: https://doi.org/10.20546/ijcmas.2017.612.033


Autolysis of Rice Bran Phytate in Long-Term Study on Batch Fermentor
Abd-ElAziem Farouk, N. Thoufeek Ahamed, Anis Shobirin Meor Hussin and Othman AlZahrani 
1Molecular Biotechnology Research Unit, Department of Biotechnology, Faculty of Science, Taif University, Al-Hawiya 888, Kingdom of Saudi Arabia
2Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
3Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
4Department of Biology, Faculty of Science, Taif University,Al-Hawiya 888, Kingdom of Saudi Arabia
*Corresponding author
Abstract:

Microorganisms especially bacteria produce a diverse of phytate-degrading enzymes. Rice bran is excellent media for bacterial growth and enzymes secretion. The aim of the study was autolysis of rice bran phytate (6%) in long-term on batch fermentor (with constant agitator speed (300 rpm) and fixed air flow rate (0.5kg/cm2). The phytase production in the fermentor was with gradual color change from initial light green to dense green during the fermentation processes. The pH and temperature changes during production of phytase in the rice bran media over 10 weeks were observed. Initial 3 weeks, a reduction in pH from pH 6 to pH 4.2. After the middle of 4th week and 5th week considerable increase in pH towards the neutral range was observed i.e. from pH 6.2 to pH 6.99. In the 5th and 6th weeks the pH range was found to be pH 7 to pH 7.9. Starting from the beginning of 8th week to 10th week pH was in the near alkaline range pH 8- pH 8.2. The temperature of the media during the initial stages of fermentation for first 3 weeks was 22-25°C. Increase in temperature was noticed after the end of the third week. The remaining weeks from 3 to 10 the temperature range was 25°C-29°C. The temperature of the media inside the fermentor was in between 22°C and 29°C throughout the study (environment temperature 20-40°C). Enzymatic partitional hydrolyzed of rice bran phytate into lower myoinsitol phosophates will have many health benefits applications.


Keywords: Autolysis, Fermentation, Phytate-degrading enzymes, Rice bran.
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How to cite this article:

Abd-ElAziem Farouk, N. Thoufeek Ahamed, Anis Shobirin Meor Hussin and Othman AlZahrani. 2017. Autolysis of Rice Bran Phytate in Long-Term Study on Batch Fermentor.Int.J.Curr.Microbiol.App.Sci. 6(12): 266-274. doi: https://doi.org/10.20546/ijcmas.2017.612.033