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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 53-65
DOI: https://doi.org/10.20546/ijcmas.2017.612.007


Chickpea: No More a Recalcitrant Species for in vitro Regeneration Response
Suma S. Biradar1*, O. Sridevi1, P.M. Salimath2, P.U. Krishnaraj3, S.A. Desai1,V. Rudra Naik1, Guruprasad Hiremath1 and B.A. Veeresha1
1Department of Genetics and plant breeding, UAS, Dharwad-580005, Karnataka, India
2UAS, Raichur, Karnataka, India
3Institute of Agricultural biotechnology, UAS, Dharwad-580005, Karnataka, India
*Corresponding author
Abstract:

In the present investigation, various factors influencing in vitro response were studied to develop an efficient reproducible regeneration protocol. Culture conditions for various stages of adventitious shoot regeneration including the induction, elongation and rooting of the elongated shoots were optimized. All the three genotypes exhibited varied response to explant type, kind and concentration of growth regulators and also recorded high degree of interaction effect. Apart from the shoot number, regeneration of quality shoots was considered by using combination of cytokinins. Good success was achieved for root regeneration and establishment of seedlings. Thus, the present study open ups the new horizons for genetic manipulation of recalcitrant pulse crops like chickpea through biotechnological interventions.


Keywords: Chickpea, Axillary meristem explants, Rooting, Recalcitrant.
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How to cite this article:

Suma S. Biradar, O. Sridevi, P.M. Salimath, P.U. Krishnaraj, S.A. Desai, V. Rudra Naik, Guruprasad Hiremath and Veeresha, B.A. 2017. Chickpea: No More a Recalcitrant Species for in vitro Regeneration Response.Int.J.Curr.Microbiol.App.Sci. 6(12): 53-65. doi: https://doi.org/10.20546/ijcmas.2017.612.007