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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3685-3693
DOI: https://doi.org/10.20546/ijcmas.2017.611.431


Characterization of Microbial Lipid Production with  Mucor rouxii on Pure Carbon Substrates
Iniya Kumar Muniraj*, Liwenxiao and Xinmin Zhan
Department of Civil Engineering, College of Engineering and Informatics National University of Ireland Galway, Galway
*Corresponding author
Abstract:

Oleaginous mould Mucor rouxii was cultivated in the medium containing glucose, starch and cellulose with an initial C/N ratio of 60. The biochemical behaviors of Mucor rouxii were examined: The highest lipid yield (4.9 g/L) was found with glucose as the carbon substrate. Starch was good for biomass production (15.5 g biomass/L medium). The Lipid content in biomass with starch as the carbon substrate was less than glucose (25%). The maximum lipid yield was increased (up to 5.8 g/L) with increasing the starch concentration to 60 g/L. Cellulose did not support lipid production. Significant quantities of α-amylase (0.5 and 1.2 IU/mL) and cellulase (0.19 IU/mL) were produced. The research suggest that in order to consume complex carbon substrates oleaginous mold should secrete complex enzymes to break down the substrates into simpler sugars and channelize them for lipid production. This study is one of the first in utilizing cellulose as a carbon source for lipid and Gamma Linoleic Acid (GLA) accumulation. The content of GLA varied considerably with the substrates.


Keywords: Lipid, Oleaginous mold, Mucor rouxii, Hydrolytic enzymes, GLA production.
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How to cite this article:

Iniya Kumar Muniraj, Liwenxiao and Xinmin Zhan. 2017. Characterization of Microbial Lipid Production with Mucor rouxii on Pure Carbon Substrates.Int.J.Curr.Microbiol.App.Sci. 6(11): 3685-3693. doi: https://doi.org/10.20546/ijcmas.2017.611.431