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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3666-3673
DOI: https://doi.org/10.20546/ijcmas.2017.611.429


Isolation and Optimization of Phytase from Pseudomonas aeruginosa and Aspergillus niger Isolated from Poultry Faeces
Faith O. Ogbonna1, Mohammed A. Milala2, Mohammad Abubakar2 and Bulama Burah2*
1Department of Biochemistry, Faculty of Science, Gombe State University, Gombe, Nigeria
2Department of Biochemistry, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
*Corresponding author
Abstract:

Phytases are enzymes that can hydrolyze phytic acid to less phosphorylated myo-inositol derivatives, releasing inorganic phosphate. In this study, phytase producing bacterium Pseudomonas aeruginosa and fungus Aspergillus niger isolated from poultry faeces were investigated and the production of phytase was optimized. Standard method was used to assay for Phytase activity. The time course production of phytase by P. aeruginosa showed that optimum phytase production was at 24 hours of incubation while that of A. niger was at 48 hours of incubation. Effects of agricultural substrates on phytase production by P. aeruginosa revealed the maximum activity (0.604µmol/min) of phytase with sorghum grain as agricultural substrate while maximum phytase activity (1.2µmol/min) by A. niger was obtained when millet grain was used. Effects of pH and temperature showed that optimum phytase activity from P. aeruginosa and A. niger was at pH 6 and 7 respectively while both preferred temperature of 37 ºC. Lactose was the best source of carbon for both. Yeast extract was observed as the best nitrogen for A. niger while urea for P. aeruginosa. In conclusion, The enzyme (phytase) was both thermostable and acid stable, can find applications in animal feed industry for improving nutritional status of the feed and combating environmental phosphorus pollution.


Keywords: Phytase, Phytic acid, Pseudomonas aeruginosa, Aspergillus niger and Poultry faeces.
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How to cite this article:

Faith O. Ogbonna, Mohammed A. Milala, Mohammad Abubakar and Bulama Burah. 2017. Isolation and Optimization of Phytase from Pseudomonas aeruginosa and Aspergillus niger Isolated from Poultry Faeces.Int.J.Curr.Microbiol.App.Sci. 6(11): 3666-3673. doi: https://doi.org/10.20546/ijcmas.2017.611.429