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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3621-3632
DOI: https://doi.org/10.20546/ijcmas.2017.611.424


Optimization of Arginine Deaminase Production from Indigenous  Bacterium Pseudomonas aeruginosa PS2
 
Anjana Sharma*, Kiran Bala and Islam Husain
Bacteriology Laboratory, Department of P. G. Studies and Research in Biological Science,Rani Durgavati University, Jabalpur, Madhya Pradesh, India
*Corresponding author
Abstract:

Arginine deaminase (ADI) is an important anticancer drug worldwide used in the chemotherapy of arginine-auxotrophic tumors, such as hepatocellular carcinomas and melanomas. To date, ADI obtained from Mycoplasma has been commercially used in clinics. However, low yield, high toxicity, short proteolytic and low serum tolerances are the major limitations of clinically available ADI. In the present investigation, we have described optimization of environmental and nutritional requirements for maximum biosynthesis of ADI from bacterium Pseudomonas aeruginosa PS2. We observed that batch time 25 h, inoculum age 20 h, 8% (v/v) inoculum size, pH 6.5 and temperature 37 °C were found as the most suitable operating conditions for ADI production. Galactose, peptone, KH2PO4 and L-arginine were found as the best carbon, nitrogen, mineral ion and inducer for ADI production, respectively. These results suggested that P. aeruginosa PS2 could be used for large-scale production of ADI but further studies are still required for strengthening the current findings which are underway in our lab.


Keywords: Arginine deaminase, Optimization, Pseudomonas aeruginosa, Cancer, Fermentation parameters.
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How to cite this article:

Anjana Sharma, Kiran Bala and Islam Husain. 2017. Optimization of Arginine Deaminase Production from Indigenous Bacterium Pseudomonas aeruginosa PS2.Int.J.Curr.Microbiol.App.Sci. 6(11): 3621-3632. doi: https://doi.org/10.20546/ijcmas.2017.611.424