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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3521-3535
DOI: https://doi.org/10.20546/ijcmas.2017.611.413


Computational Identification and Screening of Natural Compounds as Drug Targets against the Fish Pathogen, Pseudomonas fluorescens
S. Murali1, Shrinivas Jahageerdar2*, Sanath Kumar3 and Gopal Krishna4
1Division of Molecular Biology and Biotechnology, ICAR-NBFGR, Lucknow-226002, India
2Division of Fish Genetics and Biotechnology
3Post Harvest Technology Department, ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
4Director, ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
*Corresponding author
Abstract:

Pseudomonas fluorescens is an important fish pathogen responsible for septicemia and ulcers in many fish species. Control of this pathogen in aquaculture systems is necessary to prevent the outbreak of disease in farmed fish. In this study, the complete proteomic information of different strains of P. fluorescens available in public domains was used for in silico subtractive analysis against zebrafish proteome to identify putative drug targets in P. fluorescens. The proteome set of P. fluorescens consisted of 32,664 proteins of which 7,794 were identified as non-paralogous protein sequences. A total of 163 essential proteins were identified in P. fluorescens with the majority of the proteins being involved in glycan biosynthesis and genetic information processing pathway. In the present study, two outer membrane proteins (OMPs), a surface antigen-D15 and a fimbrial biogenesis outer membrane usher protein, were identified as putative drug targets. A total of 2885 natural compounds available in ZINC database were virtually screened to identify their efficacy as putative drugs against the identified targets. Five natural compounds were identified, which bind to the putative drug targets and interfere with their functions. These compounds may be potentially useful as prophylactic and chemotherapeutic agents against P. fluorescence in aquaculture systems.


Keywords: Pseudomonas fluorescens, Drug targets, in silico, Virtual screening, Natural ligand compounds.
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How to cite this article:

Murali, S., Shrinivas Jahageerdar, Sanath Kumar and Gopal Krishna. 2017. Computational Identification and Screening of Natural Compounds as Drug Targets against the Fish Pathogen, Pseudomonas fluorescens.Int.J.Curr.Microbiol.App.Sci. 6(11): 3521-3535. doi: https://doi.org/10.20546/ijcmas.2017.611.413