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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3434-3441
DOI: https://doi.org/10.20546/ijcmas.2017.611.404


Plasmid Profile and Antimicrobial Susceptibility Pattern of Staphylococcus sp. Isolated from High Touch Areas within the University of Port Harcourt Nigeria
O.E. Agbagwa* and O.C. Ibeachu
Department of Microbiology, Faculty of Science, University of Port Harcourt Rivers State, Nigeria
*Corresponding author
Abstract:

The present study was conducted to determine and to compare the microbial population of ATM and computer keyboard within the University of Port Harcourt and also to determine the role of plasmid and resistance in Staphylococcus sp isolated from the high touch areas. A total of 70 swabbed samples was collected and cultured on different media and the organisms were identified using its colonial, morphological biochemical characteristics. The identified bacteria were subjected to antibiotic sensitivity test using the disc diffusion method. The plasmid of isolated S. aureus was cured by treatment with acridine orange. Results obtained from the study indicated that some of the ATM and the keyboards were positive for the presence of microorganisms which include Staphylococcus aureus, Streptococcus sp, Klebsiella sp, Escherichia coli, Pseudomonas sp and Micrococcus sp. The antibiogram study was carried out on the identified organisms and the result showed that majority of the bacteria were resistant to most of the standard antibiotics including cloxacillin, followed by augmentin, erythromycin, tetramycin, and streptomycin. Staphylococcus sp were subjected to plasmid profiling with the use of acridine orange to detect the role of plasmid in their resistance and its was observed that they were susceptible to chloramphenical (98%), Gentamycin (94%), cotrimoxazole (78%), streptomycin (60%), tetramycin (58%), augmentin (52%) and cloxacillin (40%) because some antibiotic markers in Staphylococcus isolates are plasmid mediated . Hand washing after ATM and computer usage should be adopted to avoid contamination.


Keywords: Antibiotics, Disc diffusion, High touch area, Plasmid profile, S. aureus.
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How to cite this article:

Agbagwa, O.E. and Ibeachu, O.C. 2017. Plasmid Profile and Antimicrobial Susceptibility Pattern of Staphylococcus sp. Isolated from High Touch Areas within the University of Port Harcourt Nigeria.Int.J.Curr.Microbiol.App.Sci. 6(11): 3434-3441. doi: https://doi.org/10.20546/ijcmas.2017.611.404