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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3252-3262
DOI: https://doi.org/10.20546/ijcmas.2017.611.381


Exploring Quorum Sensing Loci and Biofilm Formation in Bacillus Isolates from Pigeonpea Rhizosphere
K.P. Smitha*, E. Rajeswari, D. Alice and T. Raguchander
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore – 3, Tamil Nadu, India
*Corresponding author
Abstract:

The present study aimed at characterizing the quorum sensing loci of potential Bacillus strain from pigeonpea rhizosphere for combating the root rot and wilt diseases of pigeonpea. Screening of 139 Bacillus isolates from pigeonpea rhizosphere under in vitro conditions revealed that 18 were potential inhibitors of root rot and wilt pathogens. Formation of biofilm by these isolates was observed in solid and liquid media which was quantified by microtitre plate assay. The presence of quorum sensing genes (comQ, comX, comP and comA) in Bacillus strains were detected by PCR following primer designing using the Primer3 software. The ComQXP quorum sensing loci was amplified at approximately 3 kb using forward primer UniComQ and the reverse primer P1. The protein translates of the comX and comP regions of the sequenced nucleotides revealed identity with comX pheromone precursor and histidine kinase sensor protein respectively. The ComA gene of CcB7 was sequenced and the protein translates of which showed identity to response regulation transcription factor. All the sequences are deposited at the GenBank Database.


Keywords: Biocontrol, Pigeonpea, Wilt, Root rot, Quorum sensing, Biofilm.
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How to cite this article:

Smitha, K.P., E. Rajeswari, D. Alice and Raguchander, T. 2017. Exploring Quorum Sensing Loci and Biofilm Formation in Bacillus Isolates from Pigeonpea Rhizosphere.Int.J.Curr.Microbiol.App.Sci. 6(11): 3252-3262. doi: https://doi.org/10.20546/ijcmas.2017.611.381