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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3195-3201
DOI: https://doi.org/10.20546/ijcmas.2017.611.374


Occurrence of Clostridium chauvoei in Sheep and Goats in Kashmir Valley
Q. Beigh*, I. Hussain, S.A. Wani, S. Rasool, Q. Nyrah, Z.A. Kashoo, N. Nazir, A.H. Wani and S. Qureshi
Bacteriology Laboratory, Division of Veterinary Microbiology and Immunology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190006, India
*Corresponding author
Abstract:

The current study reports the occurrence of Clostridium chauvoei in sheep and goats in Kashmir valley. A total of 412 faecal samples were collected from different organized and unorganized sheep and goat farms of the Kashmir valley. The samples consisted of 212 samples from sheep (adult-70, lamb-42) and goats (adult-80, kid-20) without diarrhoea (healthy) and (adult-40, lamb-60) and goats (adult-70, kid-30) with diarrhoea, were enriched in RCM broth before final isolation on blood agar plates. Suspected haemolytic colonies were purified and were identified tentatively as C. chauvoei based on Gram’s staining and morphological characteristics, colony characteristic and biochemical tests. They were finally identified by 16S rRNA gene specific PCR. Out of the 70 adult sheep and 42 lamb samples without diarrhoea, C. chauvoei was obtained from 8(11.42%) and 10(23.8%) samples, respectively. Similarly, 5(12.5%) and 15(25%) samples from 40 adult sheep and 60 lambs with diarrhoea, respectively yielded C chauvoei. Likewise, 1(1.25%) and 2(10%) isolates were recovered from 80and 20 healthy adult goat and kid samples, respectively. The diarrhoeic samples yielded 2(12.5%) and 3(10%) isolates from adult goats and kids, respectively. The overall prevalence of C. chauvoei in sheep and goat was found to be 11.16%. Lambs and kids were found to harbour more Clostridium chauvoei than the adult animals of the corresponding species.


Keywords: Clostridium chauvoei, Faeces, Goat, Sheep and PCR.
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How to cite this article:

Beigh, Q., I. Hussain, S.A. Wani, S. Rasool, Q. Nyrah, Z.A. Kashoo, N. Nazir, A.H. Wani and Qureshi, S. 2017. Occurrence of Clostridium chauvoei in Sheep and Goats in Kashmir Valley.Int.J.Curr.Microbiol.App.Sci. 6(11): 3195-3201. doi: https://doi.org/10.20546/ijcmas.2017.611.374