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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2880-2893
DOI: https://doi.org/10.20546/ijcmas.2017.611.340


Isolation and Characterization of Rhizobium from Chickpea (Cicer arietinum)
Zeenat Wadhwa1, Vivek Srivastava2, Raj Rani1, Tanvi1, Kanchan Makkar1 and Sumit Jangra3*
1Department of Biotechnology, Dayanand College, Hisar-125001, Haryana, India
2Department of Botany, Dayanand College, Hisar-125001, Haryana, India
3Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar-125004, Haryana, India
*Corresponding author
Abstract:

Nutrient paucity in the soil poses confront to global production of food. The use of synthetic nitrogen fertilizers to boost crop yield is a recurrent farming practice, despite of its unfavorable effects and hazard to the environment and human population. Rhizobium is gram negative bacterium which associates symbiotically with the roots of leguminous plants. Screening and selecting the rhizobial strain is important for biological nitrogen fixation. The present study was aimed to isolate and identify Rhizobium from Cicer arietinum (chickpea) root nodules by using CRYEMA medium. The result indicated that a bacterium from root nodules of chickpea does not absorb red color on YEMA medium and the milky white colony with spherical convex surface was isolated. Many biochemical tests of the isolated strain like oxidase, catalase and bromothymol blue were positive, while starch hydrolysis, lipase test, lysine decarboxylase and caseinase were negative which revealed that the strain isolated from chickpea plant belongs to Rhizobia species. The study indicated that all the strains grew well at pH 6 and 7, temperature 28º C to 30º C and at salt concentration of 1%. The strains showed resistance to the antibiotics, metal salts and salinity.


Keywords: YEMA, Congo red, Chickpea, Rhizobia, Legumes and bacteria.
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How to cite this article:

Zeenat Wadhwa, Vivek Srivastava, Raj Rani, Tanvi, Kanchan Makkar and Sumit Jangra. 2017. Isolation and Characterization of Rhizobium from Chickpea (Cicer arietinum).Int.J.Curr.Microbiol.App.Sci. 6(11): 2880-2893. doi: https://doi.org/10.20546/ijcmas.2017.611.340