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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2837-2844
DOI: https://doi.org/10.20546/ijcmas.2017.611.335


Molecular Identification and Characterization of Virus Associated with Murda Complex Disease in Chilli (Cv. Byadgi Dabbi)
M. Abdul Kareem* and A.S. Byadgi
Department of Plant Pathology, University of Agricultural Sciences, Dharwad - 580 005, Karnataka, India
*Corresponding author
Abstract:

Chilli (Capsicum annuum L.) is an important crop grown worldwide for its use as spice and vegetable. Murda complex is the most destructive syndrome causing substantial economic losses in chilli production. Severe leaf curling, leaf distortion, vein banding, reduced leaf size and stunted growth are typical symptoms of the viral infection. Chilli is known to be infected by many viruses and association of Geminivirus with murda complex is under question as the complex disease doesn’t associate with whiteflies. For a reliable identification of virus associated with murda complex disease in chilli (Cv. Byadgi Dabbi), gene specific primer pairs were designed for Potyvirus (CVMV), Geminivirus (ToLCV), Tospovirus (GBNV), TMV and CMV. Upon amplification by PCR using CVMV gene specific primers, ~531 bp size amplicon was amplified in all diseased samples of chilli and no amplification was obtained in healthy samples of chilli. However, no amplification was found with ToLCV, GBNV, TMV and CMV gene specific primer pairs. Thus, the investigation focused on molecular identification, convincingly revealed Chilli veinal mottle virus (Potyvirus) association with the chilli murda complex.


Keywords: Chilli, Murda complex, Leaf curl, Virus, CVMV, GBNV, ToLCV, TMV, CMV, Byadgi Dabbi.
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How to cite this article:

Abdul Kareem, M. and Byadgi, A.S. 2017. Molecular Identification and Characterization of Virus Associated with Murda Complex Disease in Chilli (Cv. Byadgi Dabbi).Int.J.Curr.Microbiol.App.Sci. 6(11): 2837-2844. doi: https://doi.org/10.20546/ijcmas.2017.611.335