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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2651-2654
DOI: https://doi.org/10.20546/ijcmas.2017.611.311


Immunohistochemistry Study in Normally Calved Postpartum Dairy Cows
S. Alagar*, M. Selvaraju and R. Ezakial Napolean
Department of Veterinary Gynaecology and Obstetrics, Veterinary College and Research Institute, Namakkal – 2, Tamil Nadu, India
*Corresponding author
Abstract:

The puerperal uterine soundness is essential for the re-establishment of postpartum ovarian cyclicity and next pregnancy. Normally calved 36 healthy Holstein Friesian crossbred cows aged between 2nd and 5th lactations were selected immediately after parturition. Day of parturition was considered as day 0 of the experiment. All the selected cows were randomly and equally divided into 4 experimental groups viz., group I, II, III and IV (control). The endometrial tissue collected on day 10 and 30 postpartum in all the four groups through Albuchins’s biopsy catheter was processed by paraffin technique and sections were cut and mounted on coated slides for immunohistochemistry. These slides were tested for immunological expression of CD3+ and CD20+ cells to identify T and B lymphocytes. Immunohistochemistry of endometrium showed mild expression for CD 20+ cells in groups I, II and III and moderate expression of CD20 +ve cells in control cows on day 10 and 30 postpartum. None of the cows in experimental and control groups expressed positive for CD3+ cells.


Keywords: Crossbred cows, Endometrial tissue, Postpartum (PP), Immunohisto-chemistry.
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How to cite this article:

Alagar, S., M. Selvaraju and Ezakial Napolean, R. 2017. Immunohistochemistry Study in Normally Calved Postpartum Dairy Cows.Int.J.Curr.Microbiol.App.Sci. 6(11): 2651-2654. doi: https://doi.org/10.20546/ijcmas.2017.611.311