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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2569-2586
DOI: https://doi.org/10.20546/ijcmas.2017.611.302


Antibacterial Activity of Some Seaweed Extracts against Multidrug Resistant Urinary Tract Bacteria and Analysis of their Virulence Genes
Wagih A. El-Shouny*, Reda M. Gaafar, Gehan A. Ismail and Marwa M. Elzanaty
Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
*Corresponding author
Abstract:

Multidrug resistant bacteria raise a serious clinical problem for treating infectious diseases worldwide. Different seaweed algae including Ulva lactuca (Chlorophyta), Petalonia fascia (Phaeophyta) and Gelidium spinosum (Rhodophyta) were investigated as natural sources for antibacterial compounds. Different solvents ethanol, methanol, acetone and water were used to extract the antibacterial substances from these seaweeds and were examined against urinary tract multidrug resistant bacterial isolates: Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. The ethanolic extract of Ulva lactuca exhibited the most significant antibacterial activity against the tested urinary tract infection (UTI) bacterial isolates. The extract was purified and chemically analyzed using UV, IR, 1H NMR and GC-MS. The results indicated that the extract was aromatic ester derivative named Di-isooctylphthalate of molecular weight 390.56. PCR analysis of three virulence factors (adhesion, urease and hemolysin) were employed in order to genetically compare the virulence activities of the UTI isolates. The results showed that only faint PCR amplicon of hemolysin virulence gene was observed in P. mirabilis sensitive isolate, while two fragments were found in the multidrug and algal resistant corresponding isolates with ampilicon sizes of 375 and 875 bp.


Keywords: Antibacterial activity, PCR analysis, Ulva lactuca, UTI infection, Virulence genes.
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How to cite this article:

Wagih A. El-Shouny, Reda M. Gaafar, Gehan A. Ismail and Marwa M. Elzanaty. 2017. Antibacterial Activity of Some Seaweed Extracts against Multidrug Resistant Urinary Tract Bacteria and Analysis of their Virulence Genes.Int.J.Curr.Microbiol.App.Sci. 6(11): 2569-2586. doi: https://doi.org/10.20546/ijcmas.2017.611.302