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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2486-2496
DOI: https://doi.org/10.20546/ijcmas.2017.611.292


Isolation and Characterization of BpL1, a Broad Acting Lytic Bacteriophage against Brucella
Vimlesh Gupta and Hari Mohan Saxena*
Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141004, Punjab, India
*Corresponding author
Abstract:

In view of the ever increasing antimicrobial resistance, bacteriophages are promising alternatives for treatment of bacterial infections. Brucellosis is an important zoonotic disease for which currently there is no satisfactory treatment. We have isolated a new broad acting lytic brucellaphage (BpL1) from the sewage of a dairy farm. The phage lysed all the 12 Brucella abortus field isolates, B. abortus strain 99 and Brucella melitensis but did not lyse any of the heterologous species tested viz. Staphylococcus aureus, Pasteurella multocida, Escherichia coli, and Salmonella species. Streaking the lysis plaques on Brucella lawn gave clear lytic zones along the streak lines. The plaques were circular with a diameter of 0.5- 3.0 mm. At a concentration of 10-4 the phage count was 4.5 × 106 plaques per ml. It was a tailed phage with icosahedral head (62.2 nm in diameter and 73.71 nm in length), and the head to tail length was 229.21 nm. The phage belonged to the order Caudovirales and family Siphoviridae. It was inactivated within one hour at 55oC and within 4 h at -20oC. Treatment at pH 2 for 4 h and at pH 4 for 12 h inactivated it. It was also inactivated after 4 h exposure to sunlight, and within 4 min. by UV light. Chloroform and Sodium Dodecyl Sulfate inactivated it within 15 min. Lysozyme inactivated it within 1 hour whereas RNase treatment did not affect its activity.


Keywords: Brucella, Bacteriophage, Brucellaphage, Phage isolation.
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How to cite this article:

Vimlesh Gupta and Hari Mohan Saxena. 2017. Isolation and Characterization of BpL1, a Broad Acting Lytic Bacteriophage against Brucella.Int.J.Curr.Microbiol.App.Sci. 6(11): 2486-2496. doi: https://doi.org/10.20546/ijcmas.2017.611.292